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Technical Presentation

Transcript: Goals 2024 Julie Litz SMART Criteria for Goals Team Roles and Responsibilities Pro How to work out your concepts Professional Development Applying the SMART framework enables teams to create clear and actionable goals. Specificity directs focus, measurability tracks progress, achievability sets realistic expectations, relevance aligns with organizational priorities, and time-bound elements promote timely completion. Clearly defined roles and responsibilities are vital for successful implementation. Each team member should be aware of their specific duties, which fosters accountability and collaboration, reducing overlaps and gaps in task fulfillment. Goal Setting Strategies Monitoring and Evaluation Future Outlook and Trends Communication Plan Prioritization of Technical Initiatives Tracking Progress Key Performance Indicators Resource Allocation Conclusion and Next Steps Effective goal setting is crucial for aligning technical teams with organizational objectives. Utilizing structured frameworks like SMART criteria ensures that goals are specific, measurable, achievable, relevant, and time-bound, fostering clarity and direction. Effective monitoring and evaluation are critical to achieving technical goals. These processes provide insights into progress, facilitate informed decision-making, and ensure alignment with objectives, optimizing performance and resource allocation. Emerging technologies such as AI and automation are pivotal to our future goals. Staying ahead of these trends will allow us to leverage new tools and methodologies, ensuring we remain leaders in our field. An effective communication plan outlines how information will be disseminated among team members and stakeholders. It specifies channels, frequencies of updates, and feedback processes to ensure transparency and alignment throughout the project. Systematic prioritization ensures that technical projects align with business objectives and resource availability. Using methods like the Eisenhower Matrix assists teams in distinguishing between urgent and essential initiatives, facilitating effective decision-making. KPIs are essential for measuring the success of technical goals. Metrics such as project completion rates, resource utilization, and defect rates provide concrete evidence of performance against the stated objectives. Establish clear metrics to track progress against set goals. Use tools like dashboards and progress reports to visualize data, enabling teams to assess performance in real-time and make necessary adjustments to stay on course. Effective resource allocation involves identifying the necessary tools, budget, and personnel required for each goal. Properly allocating resources maximizes efficiency and ensures that no goal is under-resourced or neglected. Summarizing the key aspects of our technical goals and outlining actionable next steps ensures a focused path forward for the upcoming year. Action Steps for Each Goal Alignment with Organizational Vision Timeframe and Milestones Reporting Outcomes Call to Action for Team Engagement Recap of Goals Importance of Setting Goals Feedback Mechanisms Establishing goals provides direction and purpose. They act as benchmarks for evaluating progress and success, fostering accountability and motivation throughout the team. Each goal should be accompanied by specific action steps that outline precisely what needs to be done, when, and by whom. This ensures that all team members understand their tasks, timelines, and the context of their contributions. Establishing a clear timeframe within which goals should be achieved creates urgency and accountability. Identifying critical milestones allows teams to evaluate progress and make necessary adjustments to stay on track toward goal completion. Engaging every team member is essential for achieving our goals. Regular workshops, feedback sessions, and collaborative projects will motivate and empower our team to take ownership of their contributions. Technical goals must align with the overall organizational vision and mission. This congruence ensures that all technical efforts contribute to long-term sustainability and competitive advantage in the marketplace. Transparent reporting of outcomes fosters accountability and supports strategic decision-making. Regularly communicate progress and results to all stakeholders to reinforce commitment and promote knowledge sharing. This year, we aimed to enhance system security, improve software efficiency, and boost team collaboration. Each goal aligns with our company's strategic vision while promoting innovation and operational excellence. Implement structured feedback systems to gather insights from team members and stakeholders. Regular check-ins and surveys can provide valuable input for evaluating goal achievement and identifying areas for improvement. Implementation Plan Overview of Technical Objectives Stakeholder Involvement Importance of Continuous Improvement Adjustments and

Technical Presentation

Transcript: Shooting Dates (Outdoor): (Potential) Studio Set up 2 Indoor Set-up 1 (Aidan's house) We will carry portable lights and reflectors in case we need them, but most likely the daylight will be sufficient. Lots of focus pulls in the performance which we found interesting. Cuts between narrative/performance similarly to how we want to. Individual shots will be taken with both studio set-ups - LS, MS, MCU, CU (of faces and of instruments) Static shots of all types - WS of the band and individual closer shots. Outdoor Set-up 1/2 Camera movement Indoor Set-up 2 (Aidan's house) GoPro One shot at 0:25 that we might want. Shows singer as an inspirational figure Street wall Spotlights on individuals to minimise light hitting black curtain. Shooting dates (Studio): Studio Set up 1 The walls will have music/ drumming posters and there will be music magazines and boyish clothes scattered around the floor and bed. Again, we will use daylight and the street is nearby the first outdoor location. Sony V1E - we are filming indoors and outdoors. Desk Again infinite black. This will have papers and folders on it, with a noticeboard in front, also covered in papers (letters, notes etc.) Also Empty coffee cups (2/3). Acoustic guitar Electric guitar Drum kit Dolly Markers (for the floor) Microphone Official looking papers/letters Lots of stationery Rejection letters (to be made) Band audition poster (to be made) Music magazines Drumming posters Mobile phone Cup Visual references Camera Portable lights and reflectors if the lighting if the lamp on the desk is not enough. (Archway close to indoor location) The Defiance/Group 5 - Technical Presentation We plan to film takes of the song with: Bedroom Coloured spotlights (keeping consistent colours to previous set up) Lighting style at start, camera movements Saturday 9th November Sunday 17th November Saturday 23rd November This applies to full band shots, individual shots, and narrative. Street/ Lampost Infinite black background using the black curtain. - Tilts up and down - Arcing across - Zooms - Tracking Guitar shots/microphone movement shots. Not a very busy street, so filming will not be a problem. We will only use natural light here. Coloured back lights: (from left to right) - Blue for Daniel - Purple for Shobnam - Red for Aidan Friday 8th November Thursday 14th November Friday 15th November Wednesday 20th November Thursday 21st Novemeber Friday 22nd November Mise-en-scene Props list:

Technical Background

Transcript: Abir Rahman - Technical Background Presentation 7/15/2020 Introduction Abir Ashfakur Rahman Ph.D. in Biomolecular Sciences Boise State University, Boise, Idaho Introduction Current affiliation: Tulane University School of Medicine, Bix Lab Current Postdoctoral work Postdoc work Novel therapeutics for ischemic stroke Tandem transient middle cerebral and common carotid artery occlusion (tMCAO) Occlude both the common carotid artery and the middle cerebral artery of mice at the same time, for one hour. In vivo Cell lines and procedures In vitro bEnd.3 cells - Mouse Brain Endothelial Cells - Procedure: 8-hour Oxygen-Glucose Deprivation and 18-24 hour reperfusion - Readouts: Western blot and Immunocytochemistry Primary Mouse Neural Stem Cells (Neurospheres) - Neurogenesis questions: - Stem cell proliferation - Neural differentiation - Neuroblast migration Primary microglia: Morphological characterization and proteomic profiling. bEnd.3 Neurospheres Neurospheres (adhered and cells migrating) Autophagy dysfunction as a mechanism of Parkinson's Disease Doctoral work The role of the VPS35 D620N mutation Autophagy Mechanism of cellular homeostasis - Protein turnover - Mitochondrial turnover - Endoplasmic reticulum turnover - Lipids turnover and more Autophagy Source: Novus Biologicals Measuring Autophagic Flux Measuring Autophagic Flux Source: Stress Marq Biosciences and Pieurucci et al. Neuropharmacology (2017) VPS35 D620N Originally identified by two independent studies in 2011 as a causal mutation. VPS35 Williams E.T., Chen X., and Moore D.J., Journal of Parkinson’s Disease (2017). VPS35 D620N inhibits autophagy Autophagy defect We showed that autophagic flux was reduced in VPS35 D620N mutant cells We used an overexpression construct, either overexpressing the WT allele, or the D620N mutant allele. Lentivirus mediated gene transduction Cell line generation For generating the WT and VPS35 D620N mutant transgenic cells, - We used Lentivirus to deliver the transgenes - HEK 293T cells were first transfected with viral component coding DNA, along with our transgenic construct, which also contained a puromycin resistance gene - The virus containing media was then collected and administered to SH-SY5Y cells - Cells were selected by puromycin treatment and validated via PCR and sequencing Source: Sigma-Aldrich Source: Elegheert et al. Nature Protocols (2018) CRISPR/Cas9 mediated mutagenesis - Used Origene kit and Synthego gRNA, to induce a G to A point mutation at base position 1858. CRISPR/Cas9 The transcriptome is altered in the VPS35 D620N mutant background ECM receptor interaction pathway Transcriptomic changes KEGG pathway analysis revealed potential altered pathways Akt signaling pathway Proposed pathway Hyaluronan Signaling High molecular weight HA inhibited autophagy HA and Autophagy CD 44 and HMMR expression altered in VPS35 D620N HA receptors Phosphorylation of AKT AKT Phosphorylation Other previous work Other work Confocal microscopy/ fluorescent microscopy: Took a course in bioimaging Worked as core facilities intern Worked with C. elegans and with D. melanogaster Team management: - trained and worked with many undergraduate students - taught lab sections of undergraduate courses - trained fellow postdoc and lab technician tissue culture and related in vitro work Acknowledgements Acknowledgements Members of the Bix Lab: Dr. I. Joachim Biose, Dr. Amruta Narayanappa, Mr. Kyle Esteves and Dr. Greg Bix. My thesis committee: Dr. Brad Morrison, Dr. Allan Albig, Dr. Julia Oxford and Dr. Matthew Ferguson BMOL Program Director: Dr. Denise Wingett and Program administrator: Beth Gee Dr. Bill Walthall, Dr. Ananias Escalanate Dr. Maria Andreina Pacheco Delgado and so many more. Thank You Comments?

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