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Technical Presentation

Transcript: Our main objective was to score the most points overall. Kaino and Alex were more hands on. They helped create the two practice boards. They also were dealing more with the sensors and creating the line program for the maze. Ce'Dedra, Selicia, Dennisha, and put together the technical presentation including the information and photos while creating the program for another part of race. We began Robotics this year. Jacob is a freshman and mentor for the middle school. He'll be working with us today. Dead Reckoning VS. Line Follow Program dead reckoning or using a line following program. All the marbles, or simply get out of the maze. The maze itself was a challenge. How to stop the marbles from affecting the Boe-bot Learning the QTI sensors Create our own program by learning about the one given. Dirt on tape Reversed sensors Lnt@PD4 What attachment was utilized & why did we choose that design? We used what we called a beak/arrow shaped object in front of the Boe-bot. The arrow would be low enough so that it protects the sensors & the marbles wont harm them. It's also designed so the marbles would go around our Boe-bot. We wanted to put an attachment on the back of the Boe-bot just in case we have to back up to get back on track. The full attachment would be like a box shaped object including the arrow/beak in the front. Group members What Challenges did you meet & how did you overcome them? Our Approach We feel that our approach was the best because we used all of our resources, took advantage of the information that was given to us, and brainstormed all of our options as a group. In addition to using our line follow program we did not used dead reckoning. We decided to use the sensors because if using dead reckoning and we hit a marble it would throw the whole boe-bot off of track. Our Technical Presentation Why is our approach the best?

Technical Background

Transcript: Abir Rahman - Technical Background Presentation 7/15/2020 Introduction Abir Ashfakur Rahman Ph.D. in Biomolecular Sciences Boise State University, Boise, Idaho Introduction Current affiliation: Tulane University School of Medicine, Bix Lab Current Postdoctoral work Postdoc work Novel therapeutics for ischemic stroke Tandem transient middle cerebral and common carotid artery occlusion (tMCAO) Occlude both the common carotid artery and the middle cerebral artery of mice at the same time, for one hour. In vivo Cell lines and procedures In vitro bEnd.3 cells - Mouse Brain Endothelial Cells - Procedure: 8-hour Oxygen-Glucose Deprivation and 18-24 hour reperfusion - Readouts: Western blot and Immunocytochemistry Primary Mouse Neural Stem Cells (Neurospheres) - Neurogenesis questions: - Stem cell proliferation - Neural differentiation - Neuroblast migration Primary microglia: Morphological characterization and proteomic profiling. bEnd.3 Neurospheres Neurospheres (adhered and cells migrating) Autophagy dysfunction as a mechanism of Parkinson's Disease Doctoral work The role of the VPS35 D620N mutation Autophagy Mechanism of cellular homeostasis - Protein turnover - Mitochondrial turnover - Endoplasmic reticulum turnover - Lipids turnover and more Autophagy Source: Novus Biologicals Measuring Autophagic Flux Measuring Autophagic Flux Source: Stress Marq Biosciences and Pieurucci et al. Neuropharmacology (2017) VPS35 D620N Originally identified by two independent studies in 2011 as a causal mutation. VPS35 Williams E.T., Chen X., and Moore D.J., Journal of Parkinson’s Disease (2017). VPS35 D620N inhibits autophagy Autophagy defect We showed that autophagic flux was reduced in VPS35 D620N mutant cells We used an overexpression construct, either overexpressing the WT allele, or the D620N mutant allele. Lentivirus mediated gene transduction Cell line generation For generating the WT and VPS35 D620N mutant transgenic cells, - We used Lentivirus to deliver the transgenes - HEK 293T cells were first transfected with viral component coding DNA, along with our transgenic construct, which also contained a puromycin resistance gene - The virus containing media was then collected and administered to SH-SY5Y cells - Cells were selected by puromycin treatment and validated via PCR and sequencing Source: Sigma-Aldrich Source: Elegheert et al. Nature Protocols (2018) CRISPR/Cas9 mediated mutagenesis - Used Origene kit and Synthego gRNA, to induce a G to A point mutation at base position 1858. CRISPR/Cas9 The transcriptome is altered in the VPS35 D620N mutant background ECM receptor interaction pathway Transcriptomic changes KEGG pathway analysis revealed potential altered pathways Akt signaling pathway Proposed pathway Hyaluronan Signaling High molecular weight HA inhibited autophagy HA and Autophagy CD 44 and HMMR expression altered in VPS35 D620N HA receptors Phosphorylation of AKT AKT Phosphorylation Other previous work Other work Confocal microscopy/ fluorescent microscopy: Took a course in bioimaging Worked as core facilities intern Worked with C. elegans and with D. melanogaster Team management: - trained and worked with many undergraduate students - taught lab sections of undergraduate courses - trained fellow postdoc and lab technician tissue culture and related in vitro work Acknowledgements Acknowledgements Members of the Bix Lab: Dr. I. Joachim Biose, Dr. Amruta Narayanappa, Mr. Kyle Esteves and Dr. Greg Bix. My thesis committee: Dr. Brad Morrison, Dr. Allan Albig, Dr. Julia Oxford and Dr. Matthew Ferguson BMOL Program Director: Dr. Denise Wingett and Program administrator: Beth Gee Dr. Bill Walthall, Dr. Ananias Escalanate Dr. Maria Andreina Pacheco Delgado and so many more. Thank You Comments?


Transcript: S. NO. POINTS IMPLEMENTATION PLAN 1. The PP will carry out the EIA study within 10 Km area without Public Hearing, as per procedure prescribed in EIA Notification 2006 and prepare the EIA Report along with EMP Study area of 10 km radius is considered for EIA study. The topographical map of 10 km radius is given on next slide no. 10. 2. Point wise compliance of conditions of earlier "C T.O. under Air Act 1981 and Water Act, 1974 and authorization for operating a facility under HW (M&H) Rules, 1989; should be provided. The compliance of CTO vide letter no. F (Tech) / Alwar (Alwar) / 56 (1) / 2009 -2010 /1536 -1538 dated 08/07/2009 are enclosed as Annexure- VI. 3. The PP should obtain and provide the clearance from competent authority in the Inspectorate of Fire Fighting. The application for the clearance from the Inspectorate of Fire Fighting has been submitted the copy of the same is enclosed as Annexure- VII. 4. The PP should obtain and provide the clearance from the Inspector of Factories and Boilers. The copy of the clearance from the inspector of Factories and Boilers is enclosed as Annexure- VIII. 5 The PP should work out the water requirement for industrial purposes, if any and also provide details of hydraulic design of the STP and ETP and provide realistic details of PCM proposed to be installed. • Daily fresh water requirement after expansion will be 41.5 KLD. • The water balance is given in next slide no. 11 EIA/EMP TOR COMPLIANCE Conditions given in vide letters no. F1 (4)/SEIAA/SEAC-Raj/Sectt/Project/Cat.5 (f) B /(479)/12-13/944-946 dated 10.10.2012 TECHNICAL PRESENTATION OF GREEN FIELD INDUSTRIES – (EXPANSION PROJECT) # PLOT- A-319 A, MATASYA INDUSTRIAL AREA, TEHSIL- ALWAR (RAJ.). EXISTING PROPOSED TOTAL Chlorinated Paraffin Wax and Hydrochloric Acid 1500 -- 1500 ADC (Azo- Di- Carbonamide ) -- 2400 2400 DNPT (Di - Nitrosopenta Methylene Teramine) -- 500 500 Sodium Amide -- 480 480 Sodium Hydride -- 480 480 Existing project cost – Rs.18.00 lakh, Proposed project cost – Rs. 946.50 lakh, Total project cost after expansion- Rs.964.50 lakh VIOLATION CASE FOR OBTAINING ENVIRONMENTAL CLEARANCE AT THE 62nd MEETING OF THE STATE LEVEL EXPERT APPRAISAL COMMITTEE Meeting Hall, 4- Institutional Area, Jhalana Doongari, Rajasthan Pollution Control Board ,Jaipur - 302004 27th December, 2013 Study Period –Oct & Nov’ 2012 Work Order no. Nil, Dated -31/10/2011 PROJECT PROPONENT MR. VIMAL AGRAWAL (DIRECTOR) 207, Rakesh Deep Building, 11 Commercial Complex, Gulmohar Enclave, New Delhi – 110049 Ph: 011-4602110 / 46056810, E-mail- CONSULTANT ENKAY ENVIRO SERVICES PVT. LTD. (Serial no. 46 as per the List of Accredited EIA Consultant Organizations as on December 05, 2013) Corporate Office: L-G-6, Corporate Park, Gopal Bari, Ajmer Road, Jaipur Phone: - 0141-2363996, 4023996, Fax:-5118996 Email-, Website:

Technical Presentation

Transcript: We have five runs, and each run was divided based on the attachments and the closeness of missions next to each other. We used the polar system in programming the robot which is a two dimensional coordinate system where there is a distance and angle determined from a reference point. Below is a simple representation of how the polar system applies to our missions Mechanical Design Accuracy & Strength Programming Our Program mainly consists of my blocks. This is efficient because it makes our programming process easier and clearer. Using my blocks also made the program easy to understand for everyone. We can show you an example of our most important my blocks on the program. Every piece in our robot is there for a reason, which means no unnecessary additions. This assures simplicity and clarity in understanding the way the robot functions. This also means that we didn't need to perform any repairs on our robot. Also, everything is easily reached such as the motor, sensor, and charging port. Bases We used the gyro sensor to install a correction program on our robot (PID), it is essential for us to have this sensor as it increases the accuracy of our work drastically Mission Attachments Bee&Pig Mission Strategy We built bases to make sure the robot starts from the same point every single time. We considered making our bases as Equilateral Triangles to assure that the starting angle is 45° Our robot is designed in almost perfect symmetry, this contributes that all the mass and weight gets distributed perfectly. weight and mass distribution= perfect balance of the robot and a greater ability to walk straight and avoid faults Symmetry also helped us achieve a good point for the center of mass Runs Dimensions Our Smart Program Sensors In result to all the previously mentioned points as well as a testing period we did on the robot, we achieved a well put together, durable robot that reaches the exact point of ideal balance between accuracy, speed and power. Our final result was a powerful, fast, and faultless robot. Innovation My Blocks Process Simplicity We focused on using gears and making a gear box in order to be able to transfer motion and be able to move several extensions at once. General Specifications We built attachments to add to our robot in order to solve the missions in a logical and accurate way. Meet our Robot! We added three motors, two large motors for our wheels and one medium motor for the attachments. We considered balance and symmetry when choosing where to place these motors Strategy Our mission strategy consisted of linking our run orders to our attachments. So any missions that are near each other and could be done using the same attachment are on the same run. We also used an equation to calculate the rate of points that we should get per second. ( Number of points that can be earned/ the seconds we have= how many points we should gain per second= 395/150= 2.64) This way our time (2:30 minutes) is perfectly managed. Gyro Sensor Symmetry We made sure that our process didn't only consist on trial and error. We had a logical process of testing and editing aspects of our design and program. This means that our current programs and designs are the ones we began with after countless additions and developments. Two of the quotes we believe in regarding our programming: "There is always a better way" "Every time we do something again, we should do it better than the last time" Color Sensor Programming Our choice of wheels depended on trying and finding the best option, which was this one. This choice gave us a balance between the stability, accuracy, speed, and power that we need. When we saw the thick black lines on the mat, we knew that the usage of the color sensor was necessary and that it would be extremely helpful to use. Motors We have achieved a very smart program that applies to the Automatic Control System. As part of this Automatic control system we used PID correction values. After a number of tests we settled on these values for our Gyro PID: Kp: 5 Ki: 0.003 Kd: 400 Achieving this quality in our programming assured us that the mission is performed perfectly every single time without our intervention. We installed a gyro sensor as well as a color sensor so we can assure a more accurate robot that performs missions every time. Gears Detailed Specifications Our robot weighs around 903 grams. Its width and length equal 16.5 cms (square shape) and its height is 8 cms Wheels Attachments Ecobotic Technical Presentation

Technical Presentation

Transcript: Where it all begin... Peter Drucker Challenge Take Charge Accepting the Challenge The current head of Russell Reynold's ‘Los Angeles office at the time, Dennis Hightower, who graduated from Harvard with an MBA, been in the army, and has many other credentials, was put in charge of the search to hire the new European Vice President of Euro Disney establishment. In 1978, Dennis Hightower was chosen by the country manager of Disney to take the lead in building the Walt Disney Company consumer products for Europe and later other unions to expand its market, which he did in the Middle East and Africa divisions Master Plan Strategies "My role is to step back, take a global view and evaluate tradeoffs," Once decisions are made, I will wait for country manager's approval." Ordering but persuading people to go with his plan, giving them a vision that they could see themselves fitting into the picture. Squeaky-wheel-chair theory Understanding the various cultures of a different country that would complement the Disney brand. For the jobs that were created, there was not a distinct job role for each job position. Dennis had a hard time taking charge, instead he had everyone take some control of bits and pieces of his power. Trying to establish a new apparel strategy was a fail because his market was underexploited. The Italian country was not served because Italy is made up of small retailers, and none of the valid licenses catered to Italy. PROBLEMS Recommendation Solutions... -Create a firm job description for the President position. -Not biting off more than you could chew, and just build from the little they have, then create more by building a healthier relationship between the divisions. -Facilitating a cultural analysis of the European business. 4 You Can't Turn Back Time... & Just Run Back To The Past!

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