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Scientific presentation

Transcript: Presented by PERSON for COMPANY Scientific Investigation 1: Observing Observing the scene Senses are explored individually and then scenarios where multiple senses are used follow. SUBTOPIC 1 Senses are explored individually and then scenarios where multiple senses are used follow. seeing, touching, hearing, smelling, and tasting things. ... Record - to draw or write about what you see, touch, smell, hear, and taste. seeing, touching, hearing, smelling, and tasting things. Record - to draw or write about what you see, touch, smell, hear, and taste. TEXT TEXT PICTURES PICTURES SUBTOPIC 2 TIMELINE TIMELINE 2017 MAP MAP CHART Label 1 Label 2 Label 3 Label 4 CHART Create a hypothesis 2: Create a hypothesis The question comes first. Before you make a hypothesis, you have to clearly identify the question you are interested in studying. A hypothesis is a statement, not a question. Your hypothesis is not the scientific question in your project. The hypothesis is an educated, testable prediction about what will happen. Make it clear. A good hypothesis is written in clear and simple language. Reading your hypothesis should tell a teacher or judge exactly what you thought was going to happen when you started your project. Form your questions 3: Questions Some examples are: -What causes the roots of a plant to grow downward and the stem to grow upward? -What brand of mouthwash kills the most germs? -Which car body shape reduces air resistance most effectively? -What causes coral bleaching? -Does green tea reduce the effects of oxidation? -What type of building material absorbs the most sound? Conclusion: By Kylie Belt There are more steps but usually you don't go through any more of them just once, its an on going process. Video Summary

Scientific presentation

Transcript: BIOGALACTICS SCIENTIFIC PROJECT INTRODUCTION OBJECTIVES HYPOTHESIS EXPERIMENTATION RESULTS CONCLUSIONS BIBLIOGRAPHY INDEX INTRODUCTION 1 INTRODUCTION ACTUAL SITUATION INTRODUCTION: ACTUAL SITUATION Physical diseases: #Tendinitis #Cataracts #Cardiac diseases #Circulatory diseases ... "Space radiation can also cause radiation sicknesses that results in nausea, vomiting, anorexia and fatigue. You could develop degenerative tissue diseases such as cataracts, cardac and circulatory diseases." Nasa FEEDING PROBLEMS #1 #2 #3 INTRODUCTION: FEEDING PROBLEMS #1.- NEW CRAFT---------------$$$ #4 #2.- BAD PACKAGING: LOSING VITAMINS #3.- PILLS TO SUPPLY VITAMINS #4.- LACK OF CHEWING OBJECTIVE 2 AIM OBJECTIVES IMPROVE PSYCHOLOGICAL HEALTH AND STABILITY SAVE MONEY RECEIVE A COMPLETE DIET FUTURE RESEARCHES 6 FUTURE RESEARCHES Undevelopped Ideas FUTURE RESEARCHES Condensation HYPOTHESIS 3 HYPOTHESIS WHAT? UNICELLULAR MICHROALGAE FAST REPRODUCTION (SPIRULINA) VITAMIN PLANTS IN VITRO CHEW WHY? HYPOTHESIS: ADDITIONAL ADVANTAGES Miguel Valbuena "Sedling Growth" NASA and ESA HOW? HOW? LIQUID WITH MICHROALGAE LIQUID WITHOUT MICHROALGAE FILTER PISTON MICHROALGAE PISTON HOW? HOW? PLANTS: ALGAE: HOW? EXPERIMENTATION MATERIALS and METHOD 4 EXPERIMENT 1 EXPERIMENTATION: CLINOSTAT: Simulates gravity 0 EXPERIMENT 2 EXPERIMENTATION EXPERIMENT 2 EXPERIMENTATION ON CLINOSTAT DAY 1 DAY 7 WITH GRAVITY DAY 7 DAY 3 CONCLUSIONS 5 CONCLUSIONS EXPERIMENT 1 RESULTS AND CONCLUSIONS Microalgak mikroskopioagaz ikusita EXPERIMENT 1 RESULTS Day 1 Day 10 EXPERIMENT 1 RESULTS Day 1 Day 10 EXPERIMENT 2 RESULTS DAY 10 0.027 A 0.679 A DAY 1 EXPERIMENT 2 Conclusions BIBLIOGRAPHY 7 BIBLIOGRAPHY TITLE https://www.nasa.gov/hrp/elements/radiation/about https://www.ecoagricultor.com/espirulina/ http://www.ucs.louisiana.edu/~khh6430/clinostats.html https://www.verywellmind.com/color-psychology-green-2795817 https://www.hindustantimes.com/tech/growing-plants-in-space-is-tougher-than-you-think-it-is/story-5ZszLoDrPixlVf3jMPzixO.html Bibliography

Scientific Presentation

Transcript: Identification of "compulsive" rats Foot-shock sensitivity thresholds Resistance score = (mean punished rewards) (mean punished rewards + mean unpunished rewards of the last sessions) >0.43 → Compulsive <0.20 → Non-compulsive Foot-shocks 0.1mA for 3 sessions 0.2mA for 8 sessions 0.25mA for 5 sessions = 16 sessions in total 18 Punishment resistant = "Compulsive" (28%) 26 Punishment sensitive = "Non-compulsive" Compulsive (n = 16) and Non-compulsive (n = 20) rats mA-threshold required for observed retraction of 1, 2 or 4 paws 0.1 - 0.75mA Cue-light Lever Alcohol dispenser Materials Self-administration training Post-surgery evaluation of knockdown qPCR & Expression analysis Stereotactic surgery & Knockdown Drinking well Electric grid Punished sessions with KD-treated (n = 8) and SCR-treated compulsive rats (n = 8) 9 sessions on 0.25mA 14 sessions on 0.3mA Lever-press -> Reward + 5 sec timeout 100microL 20% EtOH per press 19 FR1- and 20 FR2-sessions Sacrifice of all Compulsive rats 50micrometer slices targeting the CeA Punchouts used for PCR TaqMan-probes AAV5-hSyn-miR EZH2-mScarlet vs. scrambled shRNA (control) 2 compulsive rats died / had seizures 1 week recovery 64 male Wistar rats 7-8 weeks old, weighing 270-280g Climate-controlled room, Handled daily Sessions every weekday for 30min Ayman, J., Palotai, M., Dochnal, R., & Bagosi, Z. (2023). Ghrelin amplifies the nicotine-induced release of dopamine in the bed nucleus of stria terminalis(Bnst). Biomedicines, 11(9), 2456. https://doi.org/10.3390/biomedicines11092456 SVT. (2023, August 23). Alkoholexperimenten: 2. Råttor med beroende. [Video]. https://www.svtplay.se/video/jwq3YVA/alkoholexperimenten/2-rattor-med-beroende?info=visa Definitions & Epidemiology Carnicella, S., Yowell, Q. V., & Ron, D. (2011). Regulation of operant oral ethanol self-administration: A dose-response curve study in rats: regulation of operant oral ethanol self-administration. Alcoholism: Clinical and Experimental Research, 35(1), 116–125. https://doi.org/10.1111/j.1530-0277.2010.01328.x Alcohol Use Disorder (AUD) - 11 criteria, e.g: Drinking more often than intended, Continuing to drink despite negative consequences, etc - Treatment prevalence in Europe only 22.3% Compulsive alcohol use - About 20% of all drug-users - Modelled as Punishment resistance World Health Organization [WHO]. (‎2018)‎. Global status report on alcohol and health 2018. World Health Organization. https://www.who.int/publications/i/item/9789241565639 Method Introduction Cue-light Lever Alcohol dispenser Neuronal Epigenetics Drinking well Electric grid Alcohol both increases and decreases methylation and acetylation, especially in the amygdala GABAergic PKCdelta+ neurons in the Central amygdala (CeA) are important in compulsive drinking The epigenetic enzyme EZH2 is upregulated in PKCd+ cells Janak, P. H., & Tye, K. M. (2015). From circuits to behaviour in the amygdala. Nature, 517(7534), 284–292. https://doi.org/10.1038/nature14188 Domi, E., Barchiesi, R., & Barbier, E. (2023). Epigenetic Dysregulation in Alcohol-Associated Behaviors: Preclinical and Clinical Evidence. Current topics in behavioral neurosciences, Advance online publication. https://doi-org.e.bibl.liu.se/10.1007/7854_2022_410 References EZH2 Transcriptional Regulation in Rodents - A mediator of compulsive alcohol use? Ponomarev, I., Wang, S., Zhang, L., Harris, R. A., & Mayfield, R. D. (2012). Gene coexpression networks in human brain identify epigenetic modifications in alcohol dependence. The Journal of Neuroscience, 32(5), 1884–1897. https://doi.org/10.1523/JNEUROSCI.3136-11.2012 Prom-Wormley, E. C., Ebejer, J., Dick, D. M., & Bowers, M. S. (2017). The genetic epidemiology of substance use disorder: A review. Drug and alcohol dependence, 180, 241–259. https://doi.org/10.1016/j.drugalcdep.2017.06.040 Qiang, M., Li, J. G., Denny, A. D., Yao, J., Lieu, M., Zhang, K., & Carreon, S. (2015). Epigenetic mechanisms are involved in the regulation of ethanol consumption in mice. International Journal of Neuropsychopharmacology, 18(2). https://doi.org/10.1093/ijnp/pyu072 Roberto, M., Gilpin, N. W., & Siggins, G. R. (2012). The central amygdala and alcohol: Role of -aminobutyric acid, glutamate, and neuropeptides. Cold Spring Harbor Perspectives in Medicine, 2(12), a012195–a012195. https://doi.org/10.1101/cshperspect.a012195 Shimizu, T., Kubovcakova, L., Nienhold, R., Zmajkovic, J., Meyer, S. C., Hao-Shen, H., Geier, F., Dirnhofer, S., Guglielmelli, P., Vannucchi, A. M., Feenstra, J. D., Kralovics, R., Orkin, S. H., & Skoda, R. C. (2016). Loss of Ezh2 synergizes with JAK2-V617F in initiating myeloproliferative neoplasms and promoting myelofibrosis. The Journal of Experimental Medicine, 213(8), 1479–1496. https://doi.org/10.1084/jem.20151136 Vanderschuren, L. J., & Everitt, B. J. (2004). Drug seeking becomes compulsive after prolonged cocaine self-administration. Science, 305(5686), 1017–1019. https://doi.org/10.1126/science.1098975 Veazey, K. J., Parnell, S.

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