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Ice ice

Transcript: Working title By Anton, Ina, Luna & Matilde Introduction Introduction Goals we found interesting UN 17 SUSTAINABILITY GOALS - 3. Good wealth and wellbeing - 5. Gender equality - 8. Decent work and economic growth - 10. Reduced Inequality - 12. responsible consumption and production 3. Good health and wellbeing Goal 3.8.1 "Coverage of essential health services" Personal interest Problem statement We feel that it is frustrating, to experience people outside of the Danish psychiatric system not understanding the struggles of lived experiences inside the system. We feel there is a need to represent these issues, in a way that accurately represents the thoughts and feelings of the people in the system, while educating people outside the system How can we use autoethnographic methods, to better represent the lived experience of people in and around the Danish psychiatric system, in an interactive installation? Does this representation create an educated awareness in people who do not have first hand experience with the Danish psychiatric system? Hearing Voices of Support initiative An art installation that improves mental health Related works Photo: Ian Fowler and Glowing Bulbs, Inc. at One Art Space 2017 What kind of interaction? (constraints) Constraints - physical, how? - how is the room decorated? - how to move around in the space? - how many can experience our installation - considerations? Interaction and methods Narrowing down Interviews Bottom-up approach Methods Autoethnographic Interaction elements - video - sound Concept We want to create an interactive room with artefacts that activate video works, expressing personal lived experiences within the Danish psychiatric system. Video Video - Themes and aspects of the psychiatric system we want to show - how does the video work with the artefacts? - 30 second videos, why? What artefacts? Artefacts The relation between artefacts and people we interviewed If one artefact is lifted a video will play and if another is picked up it will overrule... What will the room look like? Space How will the room be decorated? - Straight forward - Based on artifacts - Human being What's next? What's next? Methods Methods Autoethnography Using our five themes Make use of personal connections Artefact creation Evaluation Evaluate our problem statement Of interviewees Of parcipants Evaluation Can you help us with... What do we need now? What to you think of our evaluation? Less traditional (fun) evaluation methods? How to make sure people interact?

The Ice Crystal Template

Transcript: Who is the official cousin of RNA? How does Hairpin RNA behave when frozen? A) It connects its 2 ends together B) It chops RNA up into little pieces C) It Duplicates the RNA strand D) It glues RNA molecules together What is the name of the man who froze the vial of ammonia and cyanide for 25 years? What is the Ice Crystal Template? The basis of the theory is that life originated in ice rather than the popular belief that warm, moist conditions are necessary to promote creation of life. Stanley Miller first began his quest to discover the origin of life in 1953. His early experiments were designed to simulate the environment of primitive earth and recreate the scientific beginnings of life. Using simple molecules, extreme heat and lightening Miller was able to produce amino acids, the basic structural properties needed for life development. Unknown to his fellow scientists Miller furthered his experiment to examine the effects of extreme cold on a vial filled with ammonia and cyanide. In 1997 - 25 years after freezing it - Miller retrieved his frozen test tube and found the contents of the vial had changed from a colorless mixture to an amber colored mixture, containing amino acids and nucleotides. The Ice Crystal Template Origins of Life Say hello to Stanley Miller (1930-2007) Grandfather of Pre biotic Chemistry DNA was traditionally thought to be the building blocks of life, but as the video said, we are stuck with a chicken and the egg type problem. DNA needs protein, and protein needs DNA, so RNA was proposed as an alternative RNA World was first mentioned as a possibility in 1962, but named RNA World by Walter Gilbert in 1986. Alexandef Vlassov discovered that RNA behaves strangely in ice. When it is frozen, the ends of the RNA enzymes connect with each other to form a closed loop "like a snake biting it's tail." Hairpin RNA is normally used to cut RNA molecules into smaller pieces. When frozen, this behaves in the opposite way and connects or glues chains of RNA together What colour did the liquid in Miller’s vial turn after 25 years? A) Turquoise B) Amber C) Purple D) White Weaknesses of Ice Crystal Template Is it scientifically possible for life to evolve in ice? By: Jenny, Shanda, Elena, Keshni -A.V. Vlassov, S.A. Kazakov, B.H. Johnston, L.F. Landweber, 2005. The RNA world on Ice: a new scenario for the emergence of RNA information. J Mol Evol. Jul 21 [Epub ahead of print] -Bernhardt, H.S. (2012). The RNA world hypothesis: the worst theory of the early evolution of life (except for all the others). Biology Direct, 7, Article number 23. -Blake, D.F., Jenniskens, P. (2001). The ice of life. Scientific American, August Issue. Retrieved from -Francis, B.R. (2015). The hypothesis that the gentic code originated in coupled synthesis of proteins and the evolutionary predecessors of nucleic acids in primitive cells. Life, 5 (1), 467-505 -Fox, D. (2008). Did life evolve in ice. Discover Magazine, February Issue. Retrieved from -Georgia Institute of Technology. (2014, June 25). Origin of life: stanley miller's forgotten experiments, analyzed. ScienceDaily. Retrieved May 24, 2015 from -Levy, M., & Miller, S. L. (1998). The stability of the RNA bases: Implications for the origin of life. Proceedings of the National Academy of Sciences of the United States of America, 95(14), 7933-7938. doi:10.1073/pnas.95.14.7933 -Reichard, P. (1993). From RNA to DNA, why so many ribonucleotide reductases? Science, 260(5115), 1773-1777. doi:10.1126/science.8511586 -Robertson, M. P. (2007). Stanley miller 1930-2007. Nature Chemical Biology, 3(8), 437. doi:10.1038/nchembio0807-437 -Simon Fraser University, n.d..Prebiotic chemistry. Retrieved from -Trinks, H., Shroder, W., Biebricher, C.K., (2005). Ice and the origin of Life. Origins of Life and Evolution of Biospheres, 35, 429-445 -Van der Gluik, P.T.S., Speijer, D. (2015). How amino acids and peptides shaped the RNA world. Life, 5 (1), 230-246 - Wikipedia (2015). Stanley Miller. Retrieved from Is there Evidence to Support the Theory? - Some scientists are suggesting that it makes more sense chemically that life was created in cold conditions, because in hot conditions organisms decay more quickly. - Cyanide is thought to be one of the early chemicals on earth but it evaporates too quickly in warmth to have any reactions - This would suggest that life started in ice, because this is the only way cyanide can have an effect. - Frozen seawater is a great mix for the creation of RNA which leads to the creation of life. -Eutectic freezing can help explain why reactions occur in ice. When ice crystals form, the crystals connect only with pure water, leaving impurities

Ice Crystal Template

Transcript: The theory was first proposed in 1972 when Stanley Miller put ammonia and cyanide in a vial. The compound changed from a colorless mixture into a amber-colored mixture. He found nucleotides and amino acids (Fox, 2008, p. 1). Researchers proposed that continually freezing and thawing the molecules could speed up the chemical reactions necessary for life (World Science, 2005, para. 16). The temperature of the ice plays a part in the RNA formation process (Trinks, Schroder, & Biebricher, 2005, p. 442). Researchers believe that on the primitive earth, “pockets of liquid could have expanded into a network of channels that mixed their contents during freeze-thaw cycles” (Fox, 2008, para. 37) Some of the strengths of the ice crystal templates are the multiple experiments that have been successful in proving they exist, as well as the multiple tests that have been conducted to prove that there is a possibility that this theory exists. It is a reasonable explanation to why the crystals form. DNA as well as other forms of nucleic acid exists in most beings and it will remain in them until the point where they no longer exist. TNA molecules can connect to other molecules easily and can change easily, also helping to prove the theory. RNA can as well, showing that it is diverse. “TNA activity. TNA can interact with a few of the same proteins that interact with DNA. Using molecular evolution technology, where possible functional segments of TNA are selected from a random set of synthesized” (Evolution News, 2012, p.1) . This theory is widely based off of DNA and other forms of protein synthesizers. Protein synthesizers are a widely studied subject. Being a widely studied subject, it makes it easier to make a connection between that and ice crystal templates. RNA molecules can be cut and spliced and used in experiments with ice to help prove the theory that the first life could have been frozen in ice. The ice crystal growth rate fluctuation between can vary between stages, making it difficult to control. “The growth of ice crystals is similar to the operation of life processes, but does not support the origin of life as described by the theory of evolution. Even the growth of an ice crystal demands a Creator” (Vardiman, L. 1990, p.8). The evidence is very subjective, there is still no significant evidence to prove that it is a theory. RNA plays a vital role in the expression of genes within the formation of life (Mandal, 2013). However, RNA does not synthesize in warm temperature (Mukai, Onodera & Yamada, 2010, p. 50). “Several basic questions remain unanswered, such as whether the reaction takes place entirely in the liquid phase or involves contributions from the ice–liquid interface, and whether concentration plays a role [the efficiency of the HPR ligation reaction argues that it does not] (Vlassov, Johnston, Landweber, Kazakov, 2004, p.14). Citations: Question: When was the theory first proposed? A Very Serendipitous Finding Question: In an experiment by Vlassov et al., researchers froze a hairpin RNA molecule, the molecules reversed and chained together end to end (Fox, 2008, para.32) Ice Crystal Template Weaknesses Continued In this experiment, what was the longest chain of RNA that was observed? Question: Instant Ice Crystals In Stanley Millers 25 yearlong experiment, what two chemicals did he keep frozen and observe? \ Strengths The ice crystal growth rate fluctuation between can vary between stages, making it difficult to control. -“The growth of ice crystals is similar to the operation of life processes, but does not support the origin of life as described by the theory of evolution. Even the growth of an ice crystal demands a Creator” (Vardiman, L. 1990, p.8). Ligation is .... a) When RNA molecules snip each other into pieces b) When life is formed in ice crystals c) When the ends of RNA molecules join together, replicate and bind to make longer chains of RNA d) The process of taking legal action RNA is self-replicating. DNA is a “chemical cousin” of RNA, which displayed that it could in fact be the basis of life (World Science, 2005, para. 5). When RNA is cooled below freezing, the chains of RNA join ends into a circle, “like a snake biting its tail” (Fox, 2008, para.32 Evidence through Experiments Question: Attwater, J., Wochner, A., Pinheiro, V. B., Coulson, A., & Holliger, P. (2010). Ice as a protocellular medium for RNA replication Nature Communications, 1 (6), 1-8 DOI: 10.1038/ncomms1076 BBC. Instant ice crystals- The secret life of ice. [Videofile]. (2011, October). Retrieved Biochemistry: Icy origins for RNA copying. (2013). Nature, 502(7472), 412. doi:10.1038/502412d Fox, D. (2008, February 1). Did Life Evolve in Ice? Discover. Retrieved November 1, 2013, from In the Beginning, Before There Was RNA, There Was TNA. (2012) Evolution News. 6, November 2013. Retrieved from:


Transcript: Yushi is basically an authentic Asian cuisine turned into a modern Asian cuisine. We get our ideas of food from different Asian countries such as China, Indonesia, Thailand, Vietnamese, Japanese, etc. We are basically a fast food chain place with the concept of an “Asian Subway/Chipotle”. Unlike these places, most of our food is freshly prepared. We receive fresh fish everyday, which are used for sushi and salads. We have just-made chilled and hot grab and go dishes. You have an option to try something different everyday due to the variety of options being provided. We review performance every 6 months. We keep tracks of their overtime. This shows that they are willing to put in extra hours for themselves as well as for the company. That shows the amount of dedication they have. Our employees are one of our top priorities. We want them to succeed as much as possible and reviewing performance is a great opportunity for them to show case their abilities and talent. How would you describe Yushi? YUSHI: ASIAN KITCHEN Date: October 26 2012 Time: 3pm Individual's name: Tenzing Jangchup Position: Human Resource Location of employment: 100 Maiden Street, New York, 10038 Contact Number: 9174344767 Email Address: BY: Dorje Sherpa what kind of training program does the company offer? Is it highly structured or more informal? How often do you review performance? In order to determine the status of an employee, performing Evaluation programs is very helpful and important. It helps the HR and the manager figure out how the employees are performing. In Yushi, Jangchup evaluates his employees once every 6 months. In my opinion it should be taking place 2-3 times every six months. There are employees who might not be able to stick with the job for 6 months. It’s a very long gap that needs to be filled with the records of the employee and their progress that they have made so far. Application -Interview -Hired on Spot -Orientation _start off your first day at work. When we first opened our restaurant in Maiden Lane, we had an orientation, which lasted for a week. One of our executive Chef Danny Lachs talked about the background information of company such as when we were created and what influenced us to open up a food chain such as Yushi. It is highly structured because we planned out the orientation before hands. We have a process of going through all the information they need to know about the job and their position. We train our employee before the restaurant operates. asd NEGATIVE ASPECTS EMPLOYEE SELECTION THE END

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Transcript: Nobody knows babies like we do! Quality products . Good Customer service. Every Kid really loves this store.. BABYLOU ABOUT US About Us BabyLou was established in 2004. It has been more than a decade since we started, where we have ensured to take care of every need and want of every child and infant under one roof, true to the caption “NO BODY KNOWS BABIES LIKE WE DO”. Our benchmark is to provide 100% customer service and satisfaction and continue to deliver the same with a wide range of toys, garments and Baby Products. Play and Create We Are Best 01 02 03 Block games Building Blocks help Kids to use their brain. PLAY TO LEARN in Crusing Adventures Our Discoveries Enjoy a sunny vacation aboard a luxury yacht with the LEGO® Creator 3in1 31083 Cruising Adventures set. This ship has all the comforts you need, including a well-equipped cabin and a toilet. Sail away to a sunny bay and take the cool water scooter to the beach. Build a sandcastle, enjoy a picnic, go surfing or check out the cute sea creatures before you head back to the yacht for a spot of fishing. Escape into the mountains Disney Little Princes in Also available for your Babies..... Also... Out of The World… Our reponsibility BABYLOU…. Our Responsibility All children have the right to fun, creative and engaging play experiences. Play is essential because when children play, they learn. As a provider of play experiences, we must ensure that our behaviour and actions are responsible towards all children and towards our stakeholders, society and the environment. We are committed to continue earning the trust our stakeholders place in us, and we are always inspired by children to be the best we can be. Innovate for children We aim to inspire children through our unique playful learning experiences and to play an active role in making a global difference on product safety while being dedicated promoters of responsibility towards children.

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