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ESTUDIO DE NEOPLASIAS HEMATOLOGICAS POR CITOMETRIA DE FLUJO

Experiencia Instituto Nacional de Enfermedades Neoplasicas (INEN), Perú: Protocolo Euroflow
by

Elizabeth Cervantes

on 29 December 2014

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Transcript of ESTUDIO DE NEOPLASIAS HEMATOLOGICAS POR CITOMETRIA DE FLUJO

ESTUDIO DE NEOPLASIAS HEMATOLOGICAS
POR CITOMETRIA DE FLUJO
Experiencia INEN: Protocolo Euroflow

Dispersión frontal
FSC

TAMAÑO
Dispersión lateral SSC
COMPLEJIDAD INTERNA
Tipo de muestra
Estudios
Médula ósea
Sangre periférica
LCR

LMA
L
EUCEMIA AGUDA
LLA B

LLA T


DLPC B (LLC, Burkitt, etc)
LINFOMA
DLPC T (ATL, etc)

DLPC NK

DISCRASIA DE CELULAS PLASMATICAS
MM, MGUS

LINFOCITOS T/B/NK
RECUENTO CD4 Y CD8
RECUENTO STEM CELL CD34+
(TAMO)
Anticuerpos

INMADURO

MADURO
TODO ST

CD34
CD45
Ly:
TdT CD99

T
cyCD3

smCD3
CD7


B
CD10

CD20

CD19 cyCD79a

smKappa
CD22

smLambda

NK

CD16 CD56
My:
CD1 17

MPO

Neut
CD16 CD10

Mono
IREM2 CD64

Baso
CD123 CD203c

Dend
CD123 HLADR

Mast
CD117 CD203c
CPlasm:
CD38 CD138
cyK cyL
PANELES base
PANELES
complementarios
Laboratorio de CF - INEN, Perú
el13abeth@gmail.com
Blga. Elizabeth Cervantes
screening Leucemia Aguda
screening Neoplasia Linfoide madura
Discrasias de Células Plasmáticas
Espectro de Absorcion
Espectro de Emisión
PROTOCOLO EUROFLOW
EJEMPLO
cyCD3
/
CD45
/
cyMPO
/
cyCD79a
/
CD34
/
CD19
/
CD7
/
smCD3
-
-/+

-

+

-/+

+
- -
FACSCanto II (3 laser) detecta 8 colores
V450/V500/FITC/PE/PerCP-Cy5.5/PE-Cy7/APC/APC-H7
CD56 PE
CD3 FITC
CD19 PE-Cy7
124 anticuerpos
MO normal
blastos de linaje linfoide B
Full transcript