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Copy of Regulation o Metallothionein gene expression in rats

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mohamed adl

on 23 March 2015

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Transcript of Copy of Regulation o Metallothionein gene expression in rats

A comparative study between two mating deigns to estimate different genetic parameters in corn Zea mays, L.
Thesis presented by
Mohamed Zakaria Mohamed EL-Diasty
Under supervision of
Prof. Dr/ Ashraf Hussein Ali Abd El-Hady
Professor and Head of the department of Genetics
Faculty of Agriculture
Mansoura University
Prof Dr/
Ali Maher Mohamed El-Adl
Professor of Genetics
Faculty of Agriculture
Mansoura University
Introduction
Aim of the work
Material and Methods
Conclusion

Corn is one of the most important source of carbohydrates for feeding both human and animals. In Egypt, as well as all other countries, corn is considered an important field crop where it is cultivated for an area over 1.80 million feddans to meet the demands of feeding human and animals
In this study, two mating designs were executed, one of them was the partial diallel crosses and the second was the line X tester mating design. In order to obtain dependable results, the two mating designs used the same inbred lines to achieve the equality of the genetic materials. The validity and accuracy of either mating design would be determined using the homogeneity test of variances which would indicate which mating design is suitable for estimating the desired genetic variance component.
Scientists in all countries gave corn breeding great concern and established programs to produce hybrids with high yielding ability. The research work to produce superior hybrids continued since the discovery of the phenomena of heterosis up till now.
Results
Corn breeders utilized different mating designs in order to obtain estimates and to determine the magnitudes of different genetic parameters. The knowledge of the magnitudes of additive and non-additive genetic variances is the important factor that dictates the breeding method which would be followed.

Since all mating designs gave estimates for the different genetic variance components, the question rises about which mating design would be more accurate and present valid estimates to those compnents
Prof. Dr.
Ashraf Hussein Ali Abd El-Hady
Prof. and Head of the Department of Genetics,
Faculty of Agriculture,
Mansoura University
Prof. Dr.
Kawther Saad Kash
Prof. of Genetics,
Faculty of Agriculture,
Mansoura University
Prof. Dr.
Ali Maher Mohamed El-Adl
Prof. of Genetics,
Faculty of Agriculture,
Mansoura University.
Examining committee
blood
Prof Dr/
Kawther Saad Kash
Professor of Genetics
Faculty of Agriculture
Mansoura University
Prof Dr/
Khalefa Abd El-Maksoud Zaied
Prof. Dr.
Mohamed Abd El-Baeth El-Sehy
Prof. of Genetics,
Faculty of Agriculture,
Alexandria University
The hybrids which were obtained from both mating designs were evaluated at two locations, Mansoura and Sohag.
This study aimed to perform a comparison between two known designs i.e. partial diallel and line x tester to evaluate them through estimating GCA and SCA. The results of these two mating designs would indicate which mating design is more accurate in estimating these parameters through executing a homogeneity test of variance
The combined analysis of variance obtained for the common two vegetative traits at Mansoura and Sohag.
The combined analysis of variance obtained for yield component traits at Mansoura and Sohag
The combined analysis of variance over both locations for vegetative traits obtained from the lines x testers mating design
The combined analysis of variance over both locations for yield component traits obtained from the line x tester mating design
Experiment
Genetic materials
2009
2008
Cultivation of the seven parental inbred lines and the three testers and hybridization of them by the two studied mating designs
Evaluation of all genotypes (parental lines, testers and the hybrids) at the two locations
Mansoura
Sohag
Studied traits
Vegetative traits
Plant height (only in Mansoura)
Ear height (in both locations)
Number of ears per plant (only in Mansoura)
Number of leaves per plant (in both locations)
Leaf area (only in Sohag)
Yield component traits
Ear weight
Number of rows/ear
Number of kernels/row
Weight of 100 kernels
Statistical analysis
In this study, several types of analysis of variance were made. The Statistical procedures used in this study were done according to the analysis of variance for Randomized Complete Blocks Design (RCBD) outlined by Cochran and Cox (1957) for both experiments to study the variation and to detect the presence of differences between genotypes. Also, to study heterosis in general.
Genetic materials
Determination of heterosis
Variance components
Genetic parameters
GCA
SCA
Heritability
Broad sense
Narrow sense
Homogeneity test
RAPD - PCR
Professor of Genetics
Faculty of Agriculture
Mansoura University
From the results of this study, we could conclude the following
Heterosis is of a common occurance in all corn hybrids
Diallel crosses and lines X testers are effecient in estimating GCA and SCA
GCA would be estimated with more validity and accurity from line x tester mating design
SCA would be more valid and accurate when estimated from partial diallel mating design
The choice of parents is of a great importance in producing high yielding hybrids
The choice of hybrid for each location is important due to genotype by environmental interaction
Choice of parents in hybrids production is of paramount importance in which closely related lines yield hybrids with less performance when compared with unrelated lines
Most yield traits appeared to be highly correlated with each other indicating that selection at one trait would also improve the other one
The estimates of general heterosis for vegetative traits from the mid parents (MP %), ranges, better parent (BP %) and the means of all F1 along with their ranges at both locations and from the combined data over both locations form the partial diallel crosses mating design.
The estimates of general heterosis for yield component traits from the mid parents (MP %), ranges, better parent (BP %) and the means of all F1 along with their ranges at both locations and from the combined data over both locations form the partial diallel crosses mating design
The estimates of general heterosis for vegetative traits from the mid parents (MP %), ranges, better parent (BP %) and the means of all F1 along with their ranges at both locations and from the combined data over both locations form line x tester crosses mating design
The mean performance of the seven parents and their 21 F1 hybrids resulted from the partial diallel crosses at both locations and from the combined data for vegetative traits.
The mean performance of the seven parents and their 21 F1 hybrids resulted from the partial diallel crosses at both locations and from the combined data for yield component traits.
The mean performance of the seven parents and their 21 F1 hybrids resulted from the line x tester crosses at both locations and from the combined data for vegetative traits.
The mean performance of the seven lines, three testers and their 21 F1 hybrids resulted from line x tester mating design at both locations and from the combined data for yield component traits.
The estimates of general heterosis for yield component traits from the mid parents (MP %), ranges, better parent (BP %) and the means of all F1 along with their ranges at both locations and from the combined data over both locations line x tester crosses mating design
The combined analyses of variance and mean squares of vegetative traits at both locations for all 21 F1hybrids resulted from the diallel crosses mating design.
The combined analyses of variance and mean squares of yield component traits at both locations for all 21 F1 hybrids resulted from the diallel crosses mating design.
The analysis of variance and mean squares of vegetative traits at both locations for all 21 F1 hybrids resulted from line x tester crosses.
The analysis of variance and mean squares of yield component traits at both locations for all 21 F1 hybrids resulted from line x tester crosses.
The GCA effects of all parents involved in the diallel crosses for vegetative traits at both locations.
The SCA effects of all 21 F1 hybrids resulted from the diallel crosses for vegetative traits at both locations.
The GCA effects of all parents involved in the diallel crosses for yield component traits at both locations.
The SCA effects of all 21 F1 hybrids resulted from the diallel crosses for yield component traits at both locations.
The GCA effects of the seven parental inbred lines involved in the line x tester crosses for vegetative traits at both locations.
The GCA effects of the three testers involved in the line x tester crosses for vegetative traits at both locations.
The SCA effects of all 21 F1 hybrids resulted from the line x tester crosses for vegetative traits at both locations.
The GCA effects of the seven parental inbred lines involved in the line x tester crosses for yield component traits at both locations.
The GCA effects of the three testers involved in the line x tester crosses for yield component traits at both locations.
The SCA effects of all 21 F1 hybrids resulted from the line x tester crosses for yield component traits at both locations.
The estimated amounts of heritability in broad and narrow senses at both locations obtained for vegetative and yield component traits from the diallel crosses.
The estimated amounts of heritability in broad and narrow senses at both locations obtained for vegetative and yield component traits from the line x tester crosses.
Test of homogeneity of variances between the diallel crosses and line x tester for vegetative traits at both locations and from the combined data over both locations.
Test of homogeneity of variances between the diallel crosses and line x tester crosses for vegetative traits at both locations and from the combined data over both locations.
The genotypic and phenotypic correlation between each pair for yield component traits obtained from data at Mansoura.
OP-A02 primer.
OP- B07 primer
OP- C04 primer
OP- D13 primer
OP- D13 primer
Phylogenetic analysis of the seven different maize inbred lines
The origin and pedigree of the seven parental inbred lines and the three testers of maize.
Thank You
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