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Staphylococcus Aureus Presentation

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by

Asian Rochester

on 26 June 2013

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Transcript of Staphylococcus Aureus Presentation

Presentation of
Unknown Organism ID

Conducted
Tests of Choice

For
S.aureus
Morphologies
Pathogenesis
Role in Disease
MRSA and VRSA
MRSA is methicillin-resistant S.aureus
community-acquired” (CA-MRSA)
About 40-60% of staph infections can be treated with methicillin
Vancomycin used as the standard treatment for MRSA
VRSA Vancomycin resistant S.aureus
antibiotics used against VRSA
Staphylococcus
Aureus

Microscopic
Colony
Gram Positive
Staphylococci
- staph=clusters
- cocci= sphere
large
creamy
gold pigmented
opaque
narrow beta hemolysis
By:
A'sian Rochester

Test #1: Catalase Test
Rationale: Looks for the presence of
the catalase enzyme
+ result - result
Test #2: Coagulase Test
Rationale: Looks for presence of
coagulase enzyme
Test #3: Mannitol Salt Agar
Rationale: Looks for ability
of S.aureus to use mannitol
with the production of acid which lowers pH of the medium
Positive: Yellow
Negative: Red
Control
Inoculated Plate
What is S.aureus???
Bacterium that is a member of the Firmicutes
Staphylococcus was first identified in 1880 in Scotland by surgeon Sir Alexander Ogston
S.aureus is apart of both the skin and anterior nares of the nasal passage's normal microbiota.
20% of the human population are long-term carriers of S.aureus.
One of the 5 Primary cause of nosocomial infections of patients during their hospital stay.
S.aureus is responsible for many infections but may also occur as a commensal
Presence of S.aureus doesnt always indicate infection
Parenteral and mucous membrane Portals of Entry
Spreads by contact, skin-to-skin, fomites, and prosthetic devices
Virulence Factors

Enzymes
Coagulase, hyaluronidase, and DNase
Exotoxins
superantigen, exfoliative toxins, and other toxins
Protein A
Staphylococcal pigments
Scalded Skin Syndrome
(SSSS)
Diagnosis

Collection of specimens
Identification of the bacteria
Confirmation of diagnosis
Rapid diagnostic tests
Identification of Toxins
Antimicrobial assay studies
Disease Symptoms
Clinical Manifestation
Causes..
boils
styes
impetigo
other superficial skin infections in humans
Infections
Bacteremia
Septicemia
pneumonia
deep abscesses
osteomyelitis
endocarditis
phlebitis
mastitis
meningitis
pus
red
swelling
high fever
chills
low blood pressure
blistering
tenderness
Treatment

Antibiotic therapy
Wound drainage
Device removal
Prevention
Infection control
human to human contact
basic hand washing techniques effective in preventing its transmission.
Ethanol has proven to be an effective topical sanitizer against MRSA.
S. aureus is killed in 1 minute at 78 °C and 10 minutes at 64 °C
S.aureus
and
Nursing
REFERENCES
http://en.wikipedia.org/wiki/Staphylococcus_aureus
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3311376/
http://www.ncbi.nlm.nih.gov/pubmed/23473661
http://emedicine.medscape.com/article/971358-clinical
http://www.medicinenet.com/staph_infection/page2.htm
http://www.ehow.com/about_5100981_staphylococcus-aureus.html
http://www.news-medical.net/health/Staphylococcus-Aureus-Diagnosis.aspx
lecture Notes
Google Search, images
YouTube
Taxonomy
of
S.aureus
D- Bacteria
K- Eubacteria
P- Firmicutes
C- Baccilli
O- Bacillales
F- Staphylococcaceae
G- Staphylococcus
S- Staphylococcus aureus
S- Methicillin-resistant S. aureus
Full transcript