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BME - Seminar Talk - NS-6

Fabrication of nanofibre scaffold in a controlled environment for tissue engineering applications
by

Nisha Sharma

on 6 August 2013

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Transcript of BME - Seminar Talk - NS-6

Electrospinning
Very efficient
Cost-effective
Versatile technique
Scaffolds
An artificial extracellelar matrices (ECM) to accommodate cells and support three-dimensional tissue regeneration
Results
Establish the reproducibility of PCL nanofibre at different relative humidity at constant temperature
Presentation Outline
Introduction
Objective
Approach
Experimental Design
Results and Conclusions
Discussion
Fabrication of nanofibre scaffold in a controlled environment for tissue engineering applications
Research Supervisor:
Dr. Wankei Wan
Co- Supervisor:
Dr. Derek Boughner
Advisory committee Members:
Dr. Wankei Wan
Dr. Derek Boughner
Dr. Jeffrey L. Hutter
Lab Members:
Wan Lab members

Introduction
Meet the needs of industrial requirements
Nano-scaffolding
Fine polymer fibers of nano scale dimensions are used, which are known as the scaffold
These scaffold acts as a support structure for the damaged cells and, help rebuild missing bone and tissue
Biocompatible
Highly porous with a large surface to the volume ratio
Mechanically strong, and malleable
An ideal scaffold
Haley, J. M.E. Sc. Thesis, UWO. Bioactive Scaffolds for Tissue Engineering. 2009
www.london-nano.com
Mendelson K, Schoen FJ. Annals Of Biomedical Engineering. 2006 34(12):1799–1819.
Funding:
Tissue engineering

Controlled production of nano fibers
Fabricating a reproducible
Quality assured nano fibers
A review on polymer nanofibers by electrospinning and their applications in nanocomposites
S. Ramakrishna el.at
Poly (-Caprolactone) (PCL) (440744, Molecular Weight of 80 kDa)
– Trifluoroethanol (TFE) (T63002, 2,2,2-TFE Reagent Plus®) - (TFE) was used as the dissolving solvent
Scanning electron microscopy
Effect of fiber diameter on tensile properties of electrospun poly(3-caprolactone) by Wong et al.
Objective
We are investigating the effect of the electrospinning in a controlled environment chamber
Poly (-Caprolactone) nanofiber
at constant temperatures of 20 C
using five different relative humidity (ranging from 30 % RH – 70%RH )
and, then evaluate the effect on the resulting average nanofiber diameter by SEM
The experiment is carried out in a custom designed and constructed split flow chamber
Days
Diameter (nm)
%RH
The results showed that the fibre diameter increases with increasing humidity
The increased rate of evaporation at low humidity levels
Effect of fiber diameter on tensile properties of electrospun poly(3-caprolactone) by Wong et al.
The Tensile modulus (or elastic modulus) increases with a decrease in fiber diameter
We can conclude that, the desirable tensile modulus can be achieved with lower humidity while fabricating the ideal scaffold
Conclusions
Aortic Heart Valve
We are working towards fabricating an ideal scaffold, which will be eventually used for tissue engineering application for designing aortic valve
Thin flaps of muscle tissue
Maintain unidirectional blood flow
Discussion
The required stiffness of the tissue engineered value should be ~ 320MPa
“Design and performance assessment of aortic heart valve for tissue engineering” Ronal Alexander Guiterrez. From Florida international University
The desirable diameter for this work will be in the range of 200nm- 400nm and can be fabricated with controlling relative humidity in the range of 50%RH - 60%RH at constant temperature of 20 C for the PCL polymer
NSERC, Scholarship
for the PhD. Program
Full transcript