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Seasonal variations of viruses and heterotrohpic nanoflagell

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on 14 August 2017

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Transcript of Seasonal variations of viruses and heterotrohpic nanoflagell

By
Eman Sabbagh

Seasonal variations of viruses and heterotrohpic nanoflagellates in a costal location at the Red Sea
1.1. Oceans and the Red Sea

70% of earth covered by oceans
Aquatic environment consists of microbes that play a wide role in diversity and distribution of microbes on the planet
Marine microbes can affect they dynamic, the food and biochemical cycles

Red Sea
is an extreme and unique aquatic system
Its ideal model to study microbial communities, in which it have specific conditions
Introduction
1.2. Viruses

Viruses
consist of nucleic acid, capsid and envelope
Virus size 2-20µm
Virus abundance 10,000-100,000,000 cell per ml
Viral lysis

is the most significant factor that lead to the loss of bacteria

It responsible for losses of 30% of primary and secondary production

Effect nutrient cycles by attacking bacterial cells

Helps nutrient to move from the lysed organisms to a pool of dissolved organic matter (DOM) and particulate organic matter (POM)

Support bacterial growth by recycling C back bacterial populations
Advisor
Proffesor. Xose Anxelu Moran

Introduction
Introduction
Viruses

have two infection mechanisms (cycles)

Lytsogenic cycle
Lytic cycle
Protists
are single cell eukaryotes
Phototrophs
Mixotrophs
Heterotrophs

Heterotrophic nanoflagellates (HNF)
Size 2-20µm
Abundance 0.6-1.2X103

Microbial food web
HNF are the main consumers of bacteria in the aquatic system

They control the transportation of bacterial production to higher tropical level

Act as a channel to transport carbon and other nutrients
Temperature
Salinity
Oxygen
environmental factors
Microbial composition is changing according to the change in environmental factors such as temperature, salinity, dissolved oxygen etc

Seasonal variations in temperature contribute to dynamic, structure and availability of viral communities at their hosts
Temperature affect activity, grazing rate and abundance
Hypothesis
Viruses and HNF abundance can change by the change in biological factors such as bacterial abundance and phytoplankton abundance, and other environmental factors such as temperature
Aim
The aim of this study is to measure the abundance of viruses and HNF by using flow cytometry in a costal location at the Red Sea
How environmental factors can affect the abundnace
Material and methods
Sampling site
Fixing and storing
Sample preparation and cytometric analysis
Viruses
Sample preparation and cytometric analysis
HNF
FAC Express
FCS Express
FACSCantoII
FACSCabtoII
Preliminary results
Cytometric analysis of viruses
Figure 3: Dot plot cytogram shows all viral populations that stained with SYBR Green
Preliminary results
Cytometric analysis of viruses
Figure 3: Dot plot cytogram shows HNF and other populations
Preliminary results
Viral abundance
Preliminary results
HNF abundance
Thank you
(C. Suttle, et al.,1993)
Full transcript