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Técnicas de Biología Molecular en Parasitología

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by

Virginia Perdomo

on 20 September 2013

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Transcript of Técnicas de Biología Molecular en Parasitología

Técnicas de Biología Molecular en Parasitología
Extracción de ADN a partir de muestras de sangre humana
Gel agarosa de los productos de PCR
Real Time PCR para Chlamydia trachomatis
Técnica de Fenol-Cloroformo
Técnica de Partículas de Sílica
Purificación desde gel del producto de la PCR de Chagas con kit GFX.
Clonado en vector pTOPO del producto de PCR. Transformación en E. coli
Análisis de los clones candidatos por Colony- PCR usando las condiciones elegidas a partir del Optimizer.
PCR para Chagas con PCR Optimizer
Día 1
Día 2
Día 3
Día 4
Controles:
- Positivo
- Negativo
- Inhibición
Controles:
- Positivo
- Negativo
- Interno
- Incog + interno
Full transcript