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THE EFFECT OF SALT ON BACTERIAL GROWTH

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Andriy Zasypkin

on 3 June 2014

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Transcript of THE EFFECT OF SALT ON BACTERIAL GROWTH

Does Bacteria grow faster with or without salt.
Hypothesis
If salt is added to the petri dish where the E­-Coli grows, then the bacteria will reproduce slower. If salt is added to the petri dish where the Halophile grows, then the bacteria will reproduce faster.
STATEMENT OF PROBLEM

Procedures
1. Clean and sterilize 12 petri dishes

2. Fill petri dishes with agar and water

3. Section off the petri dishes to produce more trials

4. And E­-Coli to petri dish (half of them ­ 6) and Halophile in the other half

5. Add 150g salt and 500ml water to beaker and stir

6. Add a thin layer of salt water to 3 of the 6 petri dishes of each of the bacteria types

7. Wait 2­3 days for the bacteria to reproduce in the incubator

8. Measure and record E­-Coli amount by counting colonies

9. Clean up work area
Materials
• Salt 150g
• Beaker
• Petri dishes x12
• Escherichia Coli
• Halophile
• Agar ­ enough for 12 petri dished
• Water
• Incubator
Conclusion
The purpose of this experiment is to determine the question, does bacteria grow faster with or without salt? The null hypotheses were refuted, thus the hypothesis were supported. This means that when salt is added to E-Coli, the bacteria will reproduce slower; whilst when salt is added to Halophile, the bacteria will reproduce faster. The reasons for the results of this experiment turning out as they have, were because of the properties of the respective bacteria. The salt most likely stopped the E­-Coli form consuming water and caused most of them to die resulting in the low number of colonies. On the other hand, as the name suggests, Halophile is a salt loving bacteria, and so reproduces better with salt, resulting in a high end number of colonies. The previous experiments in this field attained relatively the same results as this experiment. Thus there was no new information discovered for the results of this scientific research. This information is valuable to society because it shows how some bacteria can be fought with salt while as other will be attracted to it. This can help people learn to fight or prevent harmful bacteria from growing fast, while helping useful bacteria to reproduce. This experiment, as with all experiments, can be improved. Especially with better lighting, more advanced microscopes, more trials, more supplies (bacteria), better controlled conditions in the incubator, and numerous other aspects. There are no further ideas or plans for continuing this experiment. As for the experiment, nothing really went well with this experiment, mainly in the fact of our minute experience in this field and the data is very varied as shown in variance row of the statistics table.
Tables
Problem and Hypothesis
Stats and Conclusion
THE EFFECT OF SALT ON BACTERIAL GROWTH
Andriy Zasypkin and Tim Henehan
Block: 04
Graph
Photos
Full transcript