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Bacteria Growth Lab

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by

Kevin Garay

on 10 July 2015

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Transcript of Bacteria Growth Lab

Bacteria Growth Lab
What variables did you use?
The variables that we used for this lab were..
The desk
Saliva
Pen/Pencil
Bottom of shoe
How did you set up the lab?
To start off, we created a lab report to help us with the lab itself. We had an introduction to the lab, basically getting a background on what the lab is about. We also of course include materials needed for the lab and we created a hypothesis that we would later on prove right or wrong. Following the completion of the lab, we typed a conclusion to summarize how the lab went and how it could be used to apply to a different lab.
What were your final results of the lab?
Finishing the lab, our final results were...
For the Petri plate with bacteria from a pen, having no Ampicillin, just LB Broth applied to it, we did get two small dots of what may be bacteria growth. Although for the one with Ampicillin, used as our control, no results were shown.
For the petri plate with saliva, having no ampicillin, just LB Broth applied to it, we did get two small dots of what may be bacteria growth. Although for the one with ampicillin, used as our control, no results were shown.

What results were to be expected?
What were your results?
Introduction
Leading up to this experiment, we started to learn about bacteria and the history of microorganisms and microbes. We learned about a famous French chemist and microbiologist by the name of Louis Pasteur, who was known for discovering the principles of vaccination, microbial fermentation, and pasteurization. Aside from learning about Louis Pasteur, we learned about the history of microorganisms and microbes, back into the time of the Romans. We learned how the Romans were the first to create an underwater sewage system. We also learned that bacteria can grow anywhere and that it is also in the air, and as a result of that, bacteria is found everywhere. As a result of our knowledge on the background, we will perform a lab to see if bacteria is present in objects like the bottom of our shoe, pencil, saliva, and the top of our desk, and prove our hypothesis is correct.


Problem:
Is bacteria present or absent from objects such as the bottom of our shoe, pencils, saliva, and top of our desk?

Materials:
4 Petri dishes with and without ampicillin (Agar plates)
LB Broth
Sharpie
Notebook
Grid Sticker
cotton swab

Hypothesis:
If we use objects from our school that are more commonly used by people, then our results would be greater and we would get more bacteria grown on our petri plates.


Procedure:
Pour the broth inside of a petri dish and then wait for it to solidify.
Place the grid sticker on top of the petri dish and with your sharpie, divide up the dish into four sections.
Label sections in order of how you will test them
Grab your cotton swab and rub it against anywhere in your school and gather bacteria that you will use.
Then rub the swab on the section that you labeled it with and keep track of where you rubbed it on the petri dish.
Throw away swab and repeat until all 4 sections are filled in.
Record data in your notebook.



Conclusion:
In conclusion, our hypothesis was wrong. For six petri plates, no results were found and on the two petri plates that did show results, there were very few bacteria found. These results do not solve our problem because we think that the lab may have been done slightly wrong. Bacteria shows up almost everywhere and it is impossible that areas such as the bottom of our shoe, did not have any bacteria. I think if someone else were to do this experiment, they might need extra equipment to get better results. The results of this experiment were inconclusive and these results can’t be applied to real life situations. It is weird that no bacteria showed up in the many places we swabbed with the cotton swab; therefore, we concluded that the experiment had an error in one of the steps and was incomplete.

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