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Frog Dissection

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by

Kimberly Kelley

on 8 January 2015

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Transcript of Frog Dissection

Frog Dissection
Place frog in the pan.
Pin the frog.
To secure the frog for the dissection, pin each of his four limbs to the pan.
Begin the first skin incision.
Use the forceps to lift the skin midway between the rear legs of the frog. Use the scalpel to make a small, vertical cut in the lifted skin.
Place him/her in the dissection pan. The frog should be lying on his dorsal (back) side with the belly facing up.

Continue the skin incision.
Use the scissors to continue the cut up the midline of the frog, all the way up to the frog's neck. Do not cut too deeply.
Make two horizontal cuts.
Using the scissors or the scalpel, make a horizontal cut between the front legs.

Use the scissors or scalpel to make a horizontal cut just above the rear legs.
Separate the skin and muscle.
Separate the skin flaps from the muscle by picking up the flap of skin. Use the scalpel to help separate the skin from the muscle.
Pin the skin flaps
Once you have opened the flaps of skin, pin them to the dissection tray.
Begin the first muscle incision.
Use the forceps to lift the muscle. Make a small cut with the scalpel. Use the scissors to cut all of the way up to the frog's neck. Do not cut too deeply.
Turn the scissor blades to cut through the chest bones.
When you reach a point just below the front legs, turn the scissor blades sideways to cut through the bones in the chest. Cut off a piece of the chest bone. We will use this later.
Make muscle cuts and pin the muscle flaps back.
Make two horizontal cuts, just like you did with the skin.

Separate the muscle from the tissue underneath. Pin the muscle flaps to the dissecting tray
Open and pin the triangular flaps.
Fold back the triangular flaps above the front legs and pin them to the dissection pan.
Find the liver. The liver is a large, brownish colored organ. It produces bile which aids in digestion.

Which organ system is it in?

Remove the liver and place it on the paper plate with the correct organ system name.
The Liver
The Heart
Find the heart. The heart is a small, triangular shaped organ between the front legs. It pumps blood throughout the frog's body

Which organ system is it in?

Remove the heart and place it on the paper plate with the correct organ system name.
The Stomach
Find the stomach. The stomach is a "J" shaped organ.

Which organ system is it in?

Remove the stomach and place it on the paper plate with the correct organ system name.
Find the small intestine. The small intestine is where nutrients are absorbed.

Which organ system is it in?

Remove the small intestine and place it on the paper plate with the correct organ system name.
The Small Intestine
The Large Intestine
Find the large intestine. The large intestine is where water is absorbed and feces is stored.

Which organ system is it in?

Remove the large intestine and place it on the paper plate with the correct organ system name.
Find the lungs. The lungs allow for gas exchange. They help the frog take in oxygen and get rid of carbon dioxide.

Which organ system is it in?

Remove the lungs and place them on the paper plate with the correct organ system name.
The Lungs
External Structures
Skin
The skin is a thin, moist body covering for the frog. Touch the skin and note its smooth texture.

Which organ system is it in?

Write skin on the paper plate with the correct organ system name.
Tympanum
The tympanum is the frog's eardrum and is sensitive to sound waves in the air and water..

Which organ system is it in?

Write tympanum on the paper plate with the correct organ system name.
Eyes
The eyes are located on either side of the head so the frog can see the front and sides. The frog has an upper eyelid, lower eyelid and a third eyelind called the nictitating membrane. This membrane is clear and protects while still allowing the frog to see.

Which organ system is it in?

Write eyes on the paper plate with the correct organ system name.
External & Internal Nares
The external and internal nares are the frog's nostrils that allow for intake of oxygen and release of carbon dioxide.

Which organ system is it in?

Write nares on the paper plate with the correct organ system name.
external
nares
internal nares
What gender is your frog?
Before dissection, we can determine your frog's gender. Male frogs have thick adhesive thumb pads on the first toe of their front legs.
Mouth
Open the mouth of your frog and examine the inside. Notice that the tongue is sticky. Behind the tongue is the glottis which opens to the airway. Behind the glottis is the opening to the esophagus.

Which organ system is it in?

Write mouth on the paper plate with the correct organ system name.
Ovaries
If your frog is female, find the eggs. They are dark cells which are produced by the ovaries.

Which organ system is it in?

Remove the eggs and place them on the paper plate with the correct organ system name.
Testes
If your frog is male, it will have testes. The testes are tan, bean shaped organs.

Which organ system is it in?

Remove the testes and place them on the paper plate with the correct organ system name.
Kidneys
Find the kidneys. The kidneys filter waste from the frog's blood.

Which organ system is it in?

If you can find the kidneys, remove them and place them on the paper plate with the correct organ system name. If you were unable to find them, write kidneys on the paper plate with the correct organ system name.
Fat
Unlike humans, frogs don't store fat next to their skin. Frogs store winter fat in fat bodies found in the body cavity. Look in your frog and find any yellow-orange fat bodies.

Which organ system is it in?

Remove the fat and place them on the paper plate with the correct organ system name.
Examine the internal organs
What organs do you see? Spend some time looking at the internal structure of the frog. Do not remove any of the organs.
End of Day 1
We are going to wrap our frog and place it in a labeled plastic bag (don't forget the piece of bone). We will continue the dissection tomorrow.
Day 2
Carefully place your frog back on the dissecting pan. Pin the legs to the pan.

Place the piece of bone on the paper plate with the correct organ system name.
Skin Flaps
Cut off a small piece of skin flap and place it on the paper plate with the correct organ system name. Then pin the skin flaps to the dissecting tray.
Cut off a small piece of muscle flap and place it on the paper plate with the correct organ system name. Then pin the muscle flaps and triangular flaps to the dissecting tray.
Muscle Flaps
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