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Trichoderma

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by

Marretje Adriaanse

on 9 April 2013

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Transcript of Trichoderma

Priming Caterpillars Trichoderma - Choicetest
- Performance test
- Gene expression Colin, Maarten, Marretje, Sophia Nematodes Introduction Changes in plant metabolism ISR Priming
Trichoderma

Experiments





Conclusion

Relevance

Summary Trichoderma colonisation Volatiles - Development of the roots
- Localisation MYB72 - Splitroot test
- Choice test
- Preference test Length (cm) of the roots GFP Control Trichorderma-78 Splitroottest - Nematodes
- Caterpillars
- Botrytis cinerea
- Volatiles Botrytis cinerea - Scoring disease
- Gene expression Choicetest Performance test Gene expression (qPCR) Genes:
- PR1
- PDF1.2
- VSP2
- Actine qPCR Caterpillar qPCR B. cinerea Scoring disease Conclusion Caterpillars Summary Generalist Relevance Mamestra brassicae Performance Scoring disease I II III Survival Pesticides


Hydroponic cultures Number of lateral roots Control T-78 Clear priming effect
T-78 most promising

Possibly useful in agriculture
Might work better then rhizobacteria

More experiments volatiles - No preference between control/trichoderma
- T-78 most effective in inhibiting caterpillar growth
- PDF 1.2 downregulated
- PR1 upregulated Botrytis cinerea - Decreased sickness in Trichoderma plants
- PDF 1.2 upregulated
- VSP2 strongly downregulated Volatiles - Increased visibility GFP (MYB72 ) in lateral roots
- Increase lateral roots
Biotroph (SA)
Necrotroph (JA + ABA)
Insects (JA + Ethyleen)
Housekeeping Conclusion - Decrease chemical products Trichoderma Beneficial fungus
Direct and systemic effects

T-34
T-78
Rhizobacteria: WSC4017
Full transcript