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DNA Barcoding

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joseph cross

on 19 December 2013

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Transcript of DNA Barcoding

Base Pair
a sequence of bases CODES for a GENE
DNA Double Helix
a barcode was originally developed to quickly identify products, such as those found in supermarkets
just like the numbers in a traditional barcode, we can use the base sequence in a DNA sequence as a unique identifier for different species of living organisms
The sequence of bases for a section of the CO1 gene in the one species of butterfly has a few base differences to the same gene section in a related species. This allows us to distinguish between species, based on this sequence difference
Ready to Start?
What is the Urban Barcode Project?
the urban bc project....
DNA Barcoding by Australian Students
Timeline for Experiment
Lit Review
sample collection plan
You will need
a well developed proposal

Analysing Sequence Data
sequence data is returned electronically in the form of traces
traces represent the sequence of bases(A, G,C or T)
that was obtained from your PCR product
Good reads have one single clear peak, whereas bad reads may have a number of peaks, making confident base calls at that position difficult.....
For this reason, it is a good idea to get sequencing done in both directions. The 2 sequences can then be compared and bad reads trimmed and replaced by a good read from the opposite strand
review the research that has been on on your topic e.g.
viewing traces, trimming obtaining consensus sequences and searching databases for a match to identify the species your sample comes from, can all be done on one site, choose either DNA Subway, or eBOL
Extract DNA
Perform PCR
Full transcript