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Tetrodoxin found in Puffer fish as substitute for Organochlo

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Jerome Arzadon

on 17 September 2013

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Transcript of Tetrodoxin found in Puffer fish as substitute for Organochlo

Tetrodotoxin from Pufferfish (Tetraodontidae) as pesticide for Locusts.
1. To kill and control pests in plants.
2. To be able to preserve the plants or
3. To make the pesticides more effective.
4. To prevent famine and hunger.
5. To help the farmers in protecting their plants.
6.To have healthy plants
Cool down and filter out the scleroprotein that
has been separated out, so a yellow transparent
liquid is obtained and called first lixiviated clear
Apply/ pour /drop intraperitoneally 0.4 mL of the first clear lixiviated liquid into the specimen

Grind the tissue into pieces , put into a container, and add de-ionized water in an amount equal to 1.5 times by weight of the tissue, then add acetic acid weighing 0.05-1%, preferably 0.1-0.3%, by weight of the tissue to extract TTX from the tissue more efficiently
Stir for a few hours at room temperature.
Filter the liquid using nylon filtering material with
a pore size of 100-200 meshes per square
inch and stainless steel mesh of 40-60 meshes
per square inch. Collect the filtrate, heat quickly
to 70-95° C. for about 3-25 minutes.

Research Methodology
• Puffer Fish
• Deionized water
• Acetic acid
• Nylon filtering material
• Ammonia
• Activated Charcoal • ethanol


The general purpose of the study is to
find an alternative source that serve as substitute
for the POPs in Pesticides.The subject matter is the effectivity of tetrodotoxin as substitute for POPs and the effect on other organisms and in our environment.The other coverage of our study are
the time needed to finish the study also
the financial capacity of the researchers.

It included the difference or the advantage of
it to the other form of pesticides.The age, sex
and the traits of the sample and the structure
of the bacteria present there are not needed anymore in the coverage. Also the materials present in other pesticides,this study is limited to the other usage of the tetrodotoxin in our environment.

The puffer is an eight inch fish and can swallow a quart of water. Puffer has a way of deterring predators it swallows water into high elastic stomach.They contain deadly poison called as tetrodotoxin calculated 200,00 times more powerful than curare at blocking the passage of impulses along the cause of many deaths in Japan.

Its flesh is permeated with certain alkaloids called Leucomaines combined with the other, Ptomaines that makes the puffer fish very poisonous. The toxin always start at the extremities, the first place notice is the lips then hits the diaphragm
next the lungs eventually it will go to the
brain then the person will be completely paralyzed.
Tetrodotoxin is use to kill this so called "Locusts" as an example of pesticide. Locust are probably the oldest migratory pest.DLIS is monitoring the situation based on information provided by countries which are greatly affected. Locust swarms devastate crops and cause major agricultural damage that may cause starvation.Locust depends in weather and ecological condtions.
Locusts are the most destructive pest in Africa. Each locust eat its weight in plants so a swarm eat 423 million pounds. Locust belong to the family called Acridadae. Female locust lays 9 to 158 eggs, Locust eggs hatch in about two weeks.Hoppers develop on five to six stage, Locust lives total three to five months.
Statement of the Problem
This study aims to produce an organic pesticide to kill locusts using Tetrodotoxin from Puffer Fish

Specifically, this seeks answers to the following

a) Is the amount of Tetrodotoxin present from the puffer fish sufficient enough to make an effective Pesticide?

b) What considerable concentration of Tetrodotoxin is enough to kill locusts ?

Significance of the Study
This study will be a significant aim to make an alternative component of pesticide made out of the tissues of the pufferfish and other compounds. It will help farmers to get rid of locusts specially when it is harvesting season. Also, it is more cheaper and safer to use compared to those pesticides out in the market. It will also help in the discovery of more efficient pesticide that will kill locusts, yet not harmful to humans.
Puffer Fish
c) What is the relationship between the varying amounts of Tetrodotoxin that will be diluted in water to the number of locusts that will be killed?
A) 30% Tetrodotoxin is to 70% Water
B) 50% Tetrodotoxin is to 50% Water
C) 60% Tetrodotoxin is to 40% Water

7.To have organic pesticides
8.To have cheap but effective pesticides
9.To have safer pesticides
10.To lessen the amount of puffer fish which
are dangerous to humans
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