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GROUP-4-PROJECT

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by

미란 최

on 18 September 2013

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Transcript of GROUP-4-PROJECT

VARIABLES
GROUP-4-PROJECT
Biology
Observe how the chemicals in the swimming pool water affects Staphylococcus epidermidis, a bacteria that benefit human to some extend

Nguyen, Jiyeon, Miran, Seiran
2 bottle of collecting swimming pool water from two different location
Agar plates
Microscope
Gram stain materials
Crystal violet
Grams Iodine
Alcohol
Safranin
Clean cotton swabs
Microscope slides
Big Swimming pool
Small Swimming pool
HYPOTHESIS
No Staphylococcus epidermidis existing afterward

Due to existence of chlorine in swimming pool
-Below 0.5% water absorption
-High and low temperature
-Acid-Resistant
-Antibacterial

Chemistry
Investigate the buffer capacity of
two samples of swimming pool water
from two different locations.
-Finest crystal clear glass
-Reflect light back
-Waterproof
-Easy to clean
Equipment
pH probe
laptop
Edulog software
at least three 50 ml beakers
pipette
50 ml distilled water
50 ml 0.1 M of NaOH(aq)
50 ml 0.1 M of HCl(aq)
two swimming pool water samples

Result
Result
Swimming pool water of primary has better buffer capacity
.
Procedure
1m 9800pa, 1.5m 14700pa, 1.8m 17640 pa
0.56m 5488pa
Independent
Swimming pool water collected from two different location

Dependent
The amount of Staphylococcus epidermidis survives after contact with swimming pool water.

Controlled
Staphylococcus epidermidis collected from human skin
Bacteria in the swimming pool initially 세ㄹ
EXPERIMENT EQUIPMENT
1.
Collect two bottles of swimming pool water from two different pools

2.
Take out some sample from each bottle by using clean cotton swabs and Swab them onto the agar plates and label the dishes
3.
After the bacterias had grown, take some bacteria from the "human skin agar plate" and mix them with some fresh swimming pool water from the bottles.
4.
After 1 hour, use those swimming pool water to grow bacteria

5.
When bacterias show up on the agar plates, the Gram staining procedures can begin.

Design Technology
How sustainable and flexible is our school swimming pool tile ?
GRAM STAINING PROCEDURE
agar plate
and Physics
how sustainable our school swimming pool?
Result
US $ 8.8 / Square Meter
US $ 14.8 / Square Meter
d
d
d
d
d
both found in small and big swimming pool
small swimming pool
big swimming pool
CONCLUSION
Hypothesis
pH of both swimming pool water should be under 7

The buffer region of the swimming pool should be broad in order to protect human skin.
answer the call of nature

chocolate time

"ool"

“Please keep our ool as it’s named”.
Chemistry: Our school swimming pool has acidic buffer but not basic buffer.

Biology: The chlorinating agents in our school swimming pools did not strong enough to kill most of the Staphylococcus epidermidis. It might allowed noxious bacteria to survive in the swimming pools water.

Design Technology: Secondary swimming pool tiles are sustainable and flexible to use but primary swimming pool tiles are not.
Full transcript