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Infection Biology - Virology lab

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Emil Lindström

on 18 December 2013

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Transcript of Infection Biology - Virology lab

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Splitting and counting of cells

infection: A549 cells with 5dl309

Drug treatment: BIX & TSA

DNA-isolation, RNA-isolation

Nanodrop measurement - perfomed to see quality and concentration of DNA & RNA

Making cDNA out of RNA. (Because of RNA-instability, and that qPCR only works with DNA.)

qPCR - relative quantification of viral DNA copy number

qPCR - absoulute quantification of viral and cellular mRNA.
Virology - Lab presentation
Emil Lindström & Erik Molander

Histone Deacetylase Inhibitor (TSA)

Histone Methyltransferace Inhibitor (BIX)
Adenovirus 5 dl309 - Deleted gene E3
E3 - Promotes apoptosis
Too few cells to begin with

contamination of MOCK

contaminated DNA and RNA samples

Low concentrations of DNA and RNA
Aim: To investigate the effects of the drugs TSA and BIX on infected cells
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