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Loop-Mediated Isothermal Amplification Including Video

LAMP process
by

Jen Thompson

on 1 August 2016

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Transcript of Loop-Mediated Isothermal Amplification Including Video

Components
FIP anneals to F2C region in target DNA
Extension & strand displacement by OmniAmp
Extension & strand displacement by OmniAmp
FIP end creates a hairpin loop
BIP primer anneals & is extended
Components for Step 2
Annealing of FIP
Step 1
Details

Step 2
Details

Step 3
Details

Watch an animation of LAMP:
F3 anneals to F3C region
B3 aligns to its complimentary position on the strand.
Extension & strand displacement by OmniAmp
Displaced strands generates first
dumbell shaped structure
Combine extension from annealed FIP & self-primed end.
New dumb bell shaped structure funnels back into step 2
Full transcript