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Step 1 Details

B3 aligns to its complimentary position on the strand.

Components

FIP anneals to F2C region in target DNA

Displaced strands generates first

dumbell shaped structure

F3 anneals to F3C region

BIP primer anneals & is extended

FIP end creates a hairpin loop

Extension & strand displacement by OmniAmp

Step 2 Details

Annealing of FIP

Components for Step 2

New dumb bell shaped structure funnels back into step 2

Combine extension from annealed FIP & self-primed end.

Step 3 Details

Watch an animation of LAMP:

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