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Loop-Mediated Isothermal Amplification Including Video
15
Transcript
Step 1 Details
B3 aligns to its complimentary position on the strand.
Components
FIP anneals to F2C region in target DNA
Displaced strands generates first
dumbell shaped structure
F3 anneals to F3C region
BIP primer anneals & is extended
FIP end creates a hairpin loop
Extension & strand displacement by OmniAmp
Step 2 Details
Annealing of FIP
Components for Step 2
New dumb bell shaped structure funnels back into step 2
Combine extension from annealed FIP & self-primed end.
Step 3 Details
Watch an animation of LAMP:
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