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Testing for reducing sugar

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by

Paul Sheffield

on 10 September 2013

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Transcript of Testing for reducing sugar

Testing for reducing sugar
L.O
How to test for reducing sugars
How to test for non-reducing sugars
How to produce a calibration curve
Dilutions

Test for Reducing sugars
Benedicts solution
Blue -> Green-> Orange-> Brick red
Heated in a water bath
Test for Non- Reducing sugars
You need to heat with HCl (aq) First
Why?
Sucrose is a Disaccharide made up of glucose and fructose
Draw a diagram to show the reaction
Task
you have a solution of 100 percent glucose
produce a calibration curve to test for glucose
you need dilutions
you need to use the colourimeters
Use this calibration curve to estimate
glucose in the solution "X"
compare your results with other groups
how accurate were you?
why?
Full transcript