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Blood Sampling: Techniques & Precautions.

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Kareem Sadek

on 20 July 2015

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Transcript of Blood Sampling: Techniques & Precautions.

Blood Sampling: Techniques & Precautions.
PBL 13

What is Blood Sampling?
Blood sampling refers to the collection of blood for:
Lab analysis.
Blood donation.
Blood Sampling Techniques
Equipment used for
Capillary Blood Sampling

Frequently used technique to obtain blood samples in young infants.

Often used to obtain blood samples when repeated measurements are needed.
Sites of collection:

Heel-prick method.

Finger-prick method.

Ear lobes.
Arterial Blood Sampling

Mainly used for analysis of arterial blood gases (ABG)

Identification of respiratory, metabolic, and mixed acid-base disorders.

Measurement of the partial pressures of respiratory gases involved in oxygenation and ventilation

Monitoring of acid-base status.

Quantificaion of oxyhaemoglobin.
Sites of collection

Blood donation.
To obtain a blood sample for diagnostic purposes.
Sites of collection:
Sterile gloves
Cotton balls or 2"x2" gauze pads
70% Alcohol swabs
Sharps container
Blood Sampling Precautions
Precautions during blood sampling

Wear gloves. Gloves must be changed between patients.
Used equipment must be discarded immediately after use. Never reuse needles.
Promptly clean and sterilize any location with spills of blood and other potentially infectious materials.

Steps that need to be taken following a needlestick injury:
Encourage the wound to bleed, ideally by holding it under running water.

Wash the wound using running water and plenty of soap.

Don't scrub the wound while you're washing it.

Dry the wound and cover it with a waterproof plaster.

Ask for medical advice.
Pathogens that can be transmitted during blood sampling:
Kareem Sadek, Nadine El-Menawy, Haidy Elghalban, Salma Osama, Ahmed Kheir Ikhwan,
Amirah Safian and Alaa Samir.

How to handle the needle after you have taken the blood sample
Q- The tourniquet cannot be left for more than ...
A 1 hour
B 2 min
C 1 min
D 2 hours
Q- Which of the following will be used when a serum sample is required:
B Heparin
C Sodium citrate
D Clot activator
Q-Which of the following identifiers would be appropriate to accurately identify the patient:
A Patient's name and identification number
B Patient's name and room number
C Physician's name and room number
D Patient and physician's names
Thank You
Under the supervision of Dr Mervat Mashaly
Equipments used in capillary
blood sampling
As those used for venipuncture, plus the following:
Proper location: Third or fourth finger of the non-dominant hand
Blood Sampling Systems
Needle and syringe system.

Evacuation system (Vacutainer system).

Butterfly system.
Median Cubital
Equipment used for Arterial Bood Sampling
As those used for venipuncture, plus the following:
Pre heparinised syringe
A container with crushed ice
Perform an Allen's test.
Safe disposal of used needles and syringes:
-Sharps dispoal containers should be available at the point of use.
-If sharps container is not immediately accessible, needles should be recapped used the one handed recapping method.

Labelling of blood samples with:
-The patient's name and identification number.
-The patient's bar code (if present).
-A biohazard sign if the blood sample contains any infectious pathogen.
Follow the same procedure outlined for venipuncture.
Expel air bubbles, cap the syringe and roll the specimen.
Place the blood sample in an iced bag, and send to the lab.
Recommended order of draw:
1-Blood culture bottle.
2-Coagulation tube (
light blue
top) contains sodium citrate for coagulation tests.
3-Clot activator (
top) for serum preparation.
4-Sodium heparin (
dark green
top) contains sodium heparin for leithium level testing.
5-EDTA (
top); contains EDTA.
6-Oxalate/fluoride (
light grey
top); contains sodium fluoride.

Advance the needle into the radial artery until a blood flashback appears, then allow the syringe to fill to the appropriate level. Do NOT pull back the syringe plunger.
Locate the radial artery using the index and middle fingers.
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