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Recombinant DNA technology

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stanley chow

on 22 November 2012

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Transcript of Recombinant DNA technology

DNA Engineering Recombinant DNA Technology How was Genetic Engineering First Invented? What is genetic engineering? Pros and Cons of Genetic Engineering Pros
Medical advances (vaccines, insulin).

Agricultural benefits (insect repellent, weather resistant, growth rate). What isn't Recombinant DNA Technology? Genetic engineering is deliberately modifying an organism's DNA using biotechnology. Artificial selection (AKA selective breeding). Sources:

http://www.buzzle.com/articles/pros-and-cons-of-genetic-engineering.html

http://ballotpedia.org/wiki/index.php/California_Proposition_37,_Mandatory_Labeling_of_Genetically_Engineered_Food_(2012)

http://www.biology-online.org/dictionary/Restriction_endonuclease

http://www.abpischools.org.uk/page/modules/geneng/geneng3a.cfm?coSiteNavigation_allTopic=1

http://www.bionetonline.org/english/content/ff_cont3.htm

http://www.biologymad.com/master.html?http://www.biologymad.com/geneticengineering/geneticengineering.htm

http://www.mnn.com/green-tech/research-innovations/photos/12-bizarre-examples-of-genetic-engineering/mad-science
http://www.ehow.com/about_5566950_benefits-recombinant-dna.html Cons
Health concerns. (allergies,long term)

Ethical Issues (human and animal cloning). rDNA is created

It modifies or adds an existing trait to the original DNA (bacteria plasmids) How Recombinant DNA Technology Works Also known as Recombinant DNA Technology. In the 1970s, Stanley Cohen collaborated with Herbert Boyer, both being interested in restriction endonucleases. In 1973, they demonstrated that a frog gene could be expressed by a bacterial cell. Proposition 37 the right to know what is in your food (labels). Voted down by 53%. Many name-brand companies lobbied against this. Genetically Modified Organisms
GMOs •The DNA has been altered. Examples are: corn, tomatoes, soy, ect.

Grapples are NOT modified organisms! • Hybrid organism of desired parent traits Not natural, but engineered When DNA is split into small segments, which is used for gene splicing in recombinant DNA technology. Restriction endonuclease: http://www.abpischools.org.uk/page/modules/geneng/geneng3a.cfm?coSiteNavigation_allTopic=1 A demonstration: Suicide Genes A gene that causes a cell to kill itself. Testing on cancer. The 4 Steps in Genetically Modifying an Organism 1) The desired trait is selected, then Restriction Enzymes cut up the DNA. 2) Gel Electrophoresis separates molecules based on their charge and size. Then the desired gene is extracted from the cell. 3) Fragment of DNA is inserted into plasmid. 4) Plating separates modified cells from ones that did not take in the new plasmid. TRUE or FALSE
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