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Characterization of a Vancomycin Intermediate-Resistant Staphylococcus aureus Isolated from a Hospital

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Syed Muzzammiluddin

on 17 September 2010

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Transcript of Characterization of a Vancomycin Intermediate-Resistant Staphylococcus aureus Isolated from a Hospital

Characterization of a Vancomycin Intermediate-ResistantStaphylococcus aureus Isolated from a Hospital VANCOMYCIN Vancomycin was first isolated in 1953 by Edmund Kornfeld (working at Eli lilly) from a soil
sample collected from the interior jungles of Borneo by a missionary. The organism that
produced it was eventually named Amycolatopsis orientalis Vancomycin is indicated for the treatment of serious, life-threatening
infections by Gram-positive bacteria that are unresponsive to other
less-toxic antibiotics. Vancomycin acts by
inhibiting proper cell
wall synthesis in
Gram-positive bacteria.
Due to the different
mechanism by which
Gram-negative bacteria
produce their cell walls
and
the various factors
related to
entering the outer
membrane
of Gram-negative
organisms,
vancomycin is not
active against
Gram-negative
bacteria Specifically, it
prevents
incorporation of
N-acetylmuramic
acid (NAM) and
N-acetylglucosamine
(NAG) peptide
subunits into
the peptidoglycan
matrix. SAMPLE Staphlococcus aureus labeled
as CP2 (MIC 16µg/ml) was
isolated from a post-operative
in-patient of local cardiac
hospital of Karachi. LAB REPORTS ß-hemolytic colonies, gram-staining,
biochemical analysis and successive
sub-culturing on selective and
differential media,
all proved that the bug is S. aureus. Cultures used:
Baird Parker agar with
egg yolk telurite (Merck)
Mannitol Salt agar (Merck)
Staphylococcus aureus
chromo agar (Oxoid)
DNase agar (Merck) Vancomycin and oxacillin
sensitivity profiles were
determined by E-test (AB-Biodisk)
broth and agar dilution methods
according to Clinical and
Laboratory Standards
Institute (CLSI) guidelines Also observed that
CP2 produced a mixture
of colonies i.e. small and
large on vancomycin
containing BHI agar MH autolysin activity analyzed
through Triton X-100 induced
autolysis assay in glycine buffer.
The organism exhibited reduced
MH activity.
Scanning electron microscope- production
of amorphous extracellular material and
undetached cells even after complete
cross wall (septa) formation Characteristics
of visa Typical characteristics of VISA seen in other
parts of the world that were also observed in
the organism isolated from this patient were High levels of oxacillin resistance (MIC >500µg/ml)
Slow growth rate and
reduced MH activity Heterogeneous type of
vancomycin resistance CP2 produced extracellular matrix material
CONCLUSION Importance of
monitoring
the usage
and also to
control of
the abuse
of antibiotics
prevention
of any further
proliferation
of this type
of notorious
bugs
I’m glad you didn’t sleep.
THANKYOU SYED MUZZAMMIL UDDIN
CLASS OF 2013
Full transcript