Loading presentation...

Present Remotely

Send the link below via email or IM

Copy

Present to your audience

Start remote presentation

  • Invited audience members will follow you as you navigate and present
  • People invited to a presentation do not need a Prezi account
  • This link expires 10 minutes after you close the presentation
  • A maximum of 30 users can follow your presentation
  • Learn more about this feature in our knowledge base article

Do you really want to delete this prezi?

Neither you, nor the coeditors you shared it with will be able to recover it again.

DeleteCancel

Make your likes visible on Facebook?

Connect your Facebook account to Prezi and let your likes appear on your timeline.
You can change this under Settings & Account at any time.

No, thanks

Chapter 3, Microbial Growth and Nutrition

No description
by

Rebekah Ward

on 26 January 2017

Comments (0)

Please log in to add your comment.

Report abuse

Transcript of Chapter 3, Microbial Growth and Nutrition

Chapter 3, Microbial Growth and Nutrition
Catabolic
Trophy.....
auto/hetero - source of carbon
photo/chemo - source of energy
chemolith/chemoorgan - source of electrons
How microbes make a living.... we can predict if a metabolism is possible....

Exergonic rxn - negative delta G, leaving the system, catabolism
Endergonic rxn - positive delta G, entering the system, anabolism
triangle = delta = "change in,"
o and ' mean under standard conditions"
products - reactants
Bioenergetics (boring name for cool subject)
Microbes employ many different strategies to meet their needs. What do they need?
Water, Carbon and energy and top tier, then other macronutrients, micronutrients
a source of electrons (often carbon, sometimes metals)
a place to dump them (any TEA O2, SO4, NO3-, fermentation = EtOH, acetate)
anabolic
molecules are built from glycolysis, Kreb's, E.D and pentose PO4 intermediates
Goal
Microbial metabolism is an exotic odyssey into the wilds of biochemistry and biophysics....

In the same way human society has a division of labor, microbial micro-habitats do too

The framework is "how to make a living"

Anabolic, Catabolic, Macro/Micronutrients
Culturing
Defined
Complex
Fastidious
Goldilocks

(the rest we know from lab)
Keeping it Real.......
Enzymes
Activation energy
Catalyst
Enzyme binds substrate(s) in active site, conformational change, reactive substrate(s) -> product
Reversible but deltaG......
coupling
Redox Rxns
Acceptor-> Reduction = adding -electrons
Donor -> Oxidation =
stealing -electrons (think aerobic respiration O2 -> H2O)

reduction potential
CAC ie Krebs
2X (pyruvate from glucose -> 3 CO2)

needs the electron carriers recycled to continue (ETC)

Biosynthetic role of intermediates
ETC into PMF into ATP
Flavins,
iron-sulfurs, cytochromes, quinones, the electrons "fall" down by Eo' and some proteins pumps H+
(H+ from NADH, water (basic and
- cell)
Diversity
Sugars
Activated glucose (UDPG, ADPG nucleotide +2 P + glucose)

Sometimes gluconeogenesis


Mostly pentose and hexose monomers

CO2 fixation
Fats
Bacteria C12-C20

Saturated / unsaturated

Glycerol + esther

Phytanyl and biphytanyl

acyl carrier protein helps construct 2C at a time
Nitrogen-Stuff
Complicated...
Proteins - glutamate, glutamine and NH4

Nucleotides - purine and pyrimidine are modified amino acids and R5P

How much? When?
Two basic levels: how fast it works, how much enzyme
Allosteric, feedback inhibition
Isoenzymes
Covalent modification (adenylation)
Genetic Regulation
we'll get heavy into it in chapter 8
Energy and Bonds
Chemical (F1)
and ion channel (F0)
components
torque

3-4 H+ -> 1 ATP

Reversible
R is the ideal gas constant = 8.314 J/mol-K
T is the absolute temperature (Kelvin)
K describes concentrations when rxn is at equilibrium
ln includes the probability of the collision of particular atoms at a certain time
Cheat Sheet
Getting the electrons to the place you need them
Shuttle, not terminal
Other ways to live
Nitrogen Fixation
N2 into NH3, requires ATP

Almost always anaerobic because of the major enzyme nitrogenase
**assumption is that pressure is always the same, geologist**
Venn
essential terms
LPS, ribosome, NAG, NAM, NAT, B 1-4, B 1-3, teichoic, lipotechoic, psuedomurien, phytanyl, biphytanyl, inner/plasma membrane, outer membrane, S layer, cell wall, porin, nucleoid, periplasm, glycolipids, glycerol esther lipids, glycerol ether lipids, flagella
Full transcript