Listeria

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by Hannah Nimo6 views·Updated Dec 4, 2020
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  1. This is my presentation for the stereo middle cytology knees.
  2. I'm going to start with a case study and then
  3. follow the specimen through the pre analytical, analytical and post
  4. analytical phases.
  5. So from a case study an 88 year old male
  6. presents with fever, nausea and headache, the patient reported a
  7. diffuse headache, accompanied by malaise, fatigue and nausea.
  8. Without vomiting, He denied confusion, irritability or a personal or
  9. family history of headaches.
  10. According to the patient, he frequently attends cookout parties and
  11. enjoys fruits, salads, wines and cheese.
  12. His vitals were normal for his blood pressure, his pulse
  13. and his breathing rate.
  14. His temperature was a little high.
  15. His complete blood count showed ah, high count for Lucas
  16. sites, and he had a positive blood culture.
  17. So normally, um, with these kind of symptoms, the doctor
  18. would request either body tissues to be collected or body
  19. fluids. So I think for this case study, blood cultures
  20. were ordered.
  21. So during the analytical phase, they would start with a
  22. gram stain of the positive blood culture, and it would
  23. show gram positive kokko bacillus.
  24. They could either be single short change or palisades the
  25. media that you can use is basically anything because it's
  26. not fastidious, so you can use she blood auger, chocolate,
  27. dogger, nutrient augur brain, heart infusion or thigh a glycol
  28. it broth.
  29. The last area like slightly increased co two incubation and
  30. the optimal growth for them is 35 degrees Celsius.
  31. You can also use cold enrichment to isolate the listeria,
  32. since it can grow in the cold and most bacteria
  33. cannot. The colony Morphology for listeria are small, round, smooth,
  34. clear colonies surrounded by a small zone of beta Hamal
  35. assist. It looked really similar to groupie struck so further
  36. into the analytical phase.
  37. The very Elin's factories for listeria are listeria license.
  38. Oh, Melissa en.
  39. It damages the fag ozone membrane, preventing the macrophage from
  40. killing bacteria.
  41. More violence factors are cattle e super oxide.
  42. This mutates fost ful, light Basie and surface protein.
  43. That's p 60 the listeria.
  44. Our cattle is positive.
  45. They hide, relies, escalate and hip, eerie their camp positive.
  46. Um and it shows like a box.
  47. Hamal Assis.
  48. Um, they're motile at room temperature Onley Not at that
  49. 35 degrees Celsius.
  50. Um, if you put them on what mounts, they'll demonstrate
  51. tumbling motility end over end.
  52. And if you put them in motility media, they'll chauhan
  53. umbrella pattern