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VIRUS DE HEPATITIS C

VHC
by

WJ Guillen Pinto

on 2 May 2013

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Transcript of VIRUS DE HEPATITIS C

Caracterización molecular del virus de la hepatitis C RT-PCR (nested-PCR) Contribuir al diagnóstico, al seguimiento de los infectados y al conocimiento de la epidemiología a través del análisis molecular determinando el genotipo y la carga viral. Metodo Trizol METODOLOGIA OBJETIVO Sketches Muestra a 175 pacientes (entre 1997 al 2001) Determinación de anticuerpo por enzima inmuno análisis Aislamiento de RNA Metodo de Trizol Amplificación del genoma del virus RT-PCR (nested-PCR) Tipificacion de VHC a través del análisis de polimorfismo de restricción y secuenciación automática RFLP`s GRACIAS Electroforesis Metodo de dideoxinucleótido electroforesis en gel de agarosa al 4% teñido con una solucion de bromuro de etidio CONCLUSIÓN 37ºC por 4 horas Fase de separación Fase de precipitación
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