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Arianne Joy E de Mesa
Hannah May R Ona
Charlene Grace S Regilme Larvicidal Effects of Crude Extracts of Rambutan (Nephelium lappaceum, L.) Peel Against Third Instar Aedes aegypti (Diptera: Culicidae) Larvae THE SUBJECTS RECOMMENDATIONS PREPARATION OF RAMBUTAN CRUDE PEEL EXTRACT LARVICIDAL ASSAY CONCLUSIONS increase the concentration of Nephelium lappaceum (rambutan) peel extract
concentrations must be at least 0.5% or 5000 ppm
MOSQUITO LARVAE typically called a wriggler
molt or shed skin and grow new ones four times in 4 to 10 days
RAMBUTAN belongs to the family Sapindaceace
a small ovate fruit closely related to lychee, pulasan, and longan.
skin of the fruit is high in tannin and saponin PROBLEMS
ADDRESSED SAPONINS freely soluble in both organic solvents and water
work by interacting with the cuticle membrane of the larvae
Is the rambutan crude peel extract an effective Aedes aegypti mosquito larvicide?
Is there a significant difference among the 5 treatments in terms of larval mortality and pupation?
Which of the 3 concentrations of rambutan peel extract (100,300, 500ppm) exhibited the highest larvicidal activity?
HYPOTHESIS A higher concentration of Rambutan crude peel extract will have a higher larvicidal effect.
SIGNIFICANCE OF THE STUDY benefit the least fortunate sector of the society in terms of cost and availabilty give farmers a new livelihood for the rambutan fruits will be purchased from them RELATED
STUDIES Deore and Khadabadi’s (2009) Findings
the crude extracts of tuber of C. borivilianum Sant. and Fernand. possess larvicidal and adult emergence inhibition activity against the mosquito A. stephensi, C. quinquefasciatus and A. aegypti
biological activity of the extracts might be due to the saponins and alkaloids that exist in plants
Govindarajan’s (2011) Findings the crude extract of Cardiospermum halicacabum Linn (Family: Sapindaceae) was a potential larvicide and ovicide for controlling C. quinquefasciatus and A. aegypti mosquitoes
extract of methanol and benzene exerted 100% mortality at 300 ppm against C. quinquefasciatus., A. aegypti attained the complete ovicidal activity at 400 ppm for the extract of methanol only GOALS test the effectivity of the rambutan crude peel extract as a mosquito larvicide
contribute an alternative larvicide to society
decrease the dengue cases in the community and in the country
METHODOLOGY Filtration of Mixture Rambutan fruits were peeled by hand
Peels were washed with distilled water, then cut into small pieces
These were extracted and macerated for 48 hours with 2 liters of ethanol
Concentration of filtrate using a Rotary Evaporator
Further concentration of filtrate using a Water Bath at 60 degrees Celsius to obtain a semi-solid extract
PREPARATION OF TREATMENTS appropriate volume of dilution for each concentration was added to 100 mL dechlorinated water contained in disposable cups
Preparation of the volume of stock solution by making 1% extract solution in ethanol
Data recordings were made every 12 hours for 48 hours after treatment application.
Transfer of 20 third instar Aedes aegypti larvae by means of disposable plastic pasteur pippettes into each test cup
Observation was extended to pupation to determine the number of pupated larvae. RESULTS LARVAL MORTALITY Table 1
Larval Mortality: Analysis of Variance
Larval Mortality: Homogeneous Subsets
LARVAL PUPATION Table 3
Larval Pupation at Day 7: Analysis of Variance Table 4
Larval Pupation at Day 7: Homogeneous Subsets Rambutan peel extract can be a substitute for synthetic mosquito larvicides against Aedes aegypti.
There is a significant difference among the five treatments.
The 500 ppm concentration exhibited the highest larvicidal activity among the three concentrations of Rambutan crude peel extract.