Loading presentation...

Present Remotely

Send the link below via email or IM


Present to your audience

Start remote presentation

  • Invited audience members will follow you as you navigate and present
  • People invited to a presentation do not need a Prezi account
  • This link expires 10 minutes after you close the presentation
  • A maximum of 30 users can follow your presentation
  • Learn more about this feature in our knowledge base article

Do you really want to delete this prezi?

Neither you, nor the coeditors you shared it with will be able to recover it again.



a tool used to move very small amounts of liquid

Cody Robinson

on 1 June 2010

Comments (0)

Please log in to add your comment.

Report abuse

Transcript of Micropipettes

what are the different colors mean? How do they work? outline http://www.le.ac.uk/ge/genie/vgec/he/micropipette_part1.html blue top = P 1000 yellow top = P 20 or P 200 clear or white top = P 2 http://www.1227.com in your spare time please check out the informational video on pipettes made by me. Not really... i wish micropipette educational videos Whats a tip? a tip is the removable end that takes in the liquid. the tip is plastic cone piece with a very small tip on one side and a large hole on the opposite side that connects the body of the pipette. the liquid is held in the body of the tip. your probably wondering how they keep all that liquid in without falling through the hole. well there a many different methods of how they work. the most common type is Piston-driven air dissplacement pipettes. Piston-driven air dissplacement pipettes Vaccum assisted pipettes the most common type of pipette These pipettes are the most precise and accurate type of pipette, they operate by piston-driven air displacement. A vacuum is generated by the vertical travel of a metal or ceramic piston within an airtight sleeve. As the piston moves upward, driven by the depression of the plunger, a vacuum is created in the space left vacant by the piston. Air from the tip rises to fill the space left vacant, and the tip air is then replaced by the liquid, which is drawn up into the tip and thus available for transport and dispensing elsewhere. These micropipette were invented and patented 1960 by Dr. Hanns Schmitz (Germany). Afterwards, the co-founder of the biotechnology company eppendorf, Dr. Heinrich Netheler, inherited the rights and initiated the global and general use of micropipettes in labs. In 1972, the adjustable micropipette was invented at the University of Wisconsin-Madison by several people, primarily inventor Warren Gilson and Henry Lardy, hence one of the bigger producers is the original company called Gilson Inc., as a result they are colloquially referred to as Gilsons Non-piston driven vacuum assisted pipettes are hollow narrow cylinders which work like a straw and require the use of some kind of additional suction device. Originally pipettes were made of pyrex glass, but currently are made of polystyrene. It is more commonly used in chemistry, with aqueous solutions. There are two types. One type, the volumetric pipette, has generally a large bulge with a long narrow portion above with a mark as it is calibrated for a single volume. Typical volumes are 10, 25, and 50 mL. Alternatively, graduated pipettes are straight-walled, and graduated for different volumes such as 5 mL in 0.5 mL increments. The single volume pipette is usually more accurate, with an error of ± 0.1 or 0.2 mL.

The pipette is filled by dipping the tip in the volume to be measured, and drawing up the liquid with a pipette filler past the inscribed mark. The volume is then set by releasing the vacuum using the pipette filler or a damp finger. While moving the pipette to the receiving vessel, care must be taken not to shake the pipette because the column of fluid may "bounce".

Positive displacement pipettes These are similar to air displacement pipettes, but are less commonly used and are used to avoid contamination and for volatile or viscous substances at small volumes, such as DNA. The major difference is that the disposable tip is a microsyringe (plastic), composed of a plunger which directly displaces the liquid.

Volumetric pipettes Volumetric pipettes allow the user to measure a volume of solution extremely accurately and then add it to something else. They are commonly used to make laboratory solutions from a base stock as well as prepare solutions for titration. They are typically marked to indicate one single volume in a particular size pipette (as are volumetric flasks). Many different sizes are available. Graduated pipettes Graduated pipettes use a series of marked lines (as on a graduated cylinder) to indicate different calibrated volumes. These also come in a variety of sizes. These are used much like a burette, in that the volume is found by calculating the difference of the liquid level before and after liquid is dispensed. Typically the precision of a graduated pipette is not as great as that of a volumetric pipette.[citation needed] Two types of graduated pipettes exist:

The tips are color coded as well. That really helps when buying and when using mutiple types of pipettes at the same time with multiple tip types aswell MICROPIPETTES a device used to move a very small amount of water. Most typical used in scientific research and in criminology. This piece of science equipment has help many researchers discover many sicknesses and cures. it is also used in microinjection. The first micropipette was patented in 1960 by Dr Hanns Schmitz (Marburg/ Germany). The founder of the company eppendorf there are many different types of micropipettes ever since 1960 a micropipette has help in almost every cure for disease involving blood, DNA, or any kind of cell disorder. How has it helped? A micropipette is... all yourn base are belong to us someone set up us the bomb mmm zig mmm zig mmm zig mmm zig mmm zig mmm zig mmm zig mmm zig mmm zig mmm zig THIS IS OVER CAUSE OH NO!!!! SOMEONE SET US UP THE BOMB!!!! up the
Full transcript