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Bacterioganic Rubber

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by

Andreas Kjær

on 8 October 2013

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Transcript of Bacterioganic Rubber

SDU-Denmark 2013
Synthesis of prenyltransferase gene
LacI/pLac promoter
araC/pBAD promoter
GFP fusion
Dxs (B. sub & E. coli)
Characterize the rubber production
To do list
How is Rubber made?
MEP pathway
The system
Characterization
Mass spectrometry (MALDI-ToF)
Static Headspace GC
Characterization of isoprene gas
Measuring MEP improvements
Summary
Optimize purification protocol for rubber
Optimize protocol for MALDI-ToF (pherhaps in-fusion ESI)
Check purification on H-NMR and MALDI-ToF
Negative effects
Terrorize rainforests
Ineffective production:
2 t/ha/yr 11,3 mio. t/yr
57000 km
7 year maturation time
20 years production
Positive aspects
Takes up less space
non-specific regional growth
Effective production
media variable
System Design
Dxs and IspG
Prenyltransferase
FACS
2
Produced in the lacitifiers of Hevea brasiliensis
Nutrient
Rubber
Glycolysis
MEP pathway
Prenyltransferase
Giard's T-reagent
2-Mercaptobenzothiazole (matrix)
iGEM
USER Cloning
Rubber Purification
Time
Concentration of rubber
Time
Concentration of B1 and B6 vitamin
Full transcript