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Lecture 1

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by

Eman Mostafa

on 8 January 2016

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Transcript of Lecture 1

Microscopy
Hist-ology

Tissue
Types of tissues
Fixation of samples
Clearing
Impregnation
Sectioning-Mounting-Staining
Common Stains
Specific Stains
Detail 3
*Definition.
*Tissue structure.
*Tissue types.
*Microscopes
*Tissue preparation.
*Staining
tissue
science
Cells
Extracellular Matrix (ECM)
epithelium
Connective tissue CT
Muscular Tissue
Nervous Tissue
Cells
Tissues
Organs
Systems
Light Microscope
Electron Microscope
Magnification
Degree of enlargement

Depends on the power of the objective and eye piece

Resolution
The least distance between two points that can be seen as two
Depends on the wave length

Electron Microscope
Electron traverse
اتالاتالا
ة
تتتتت
Electron reflect
Magnification in EM= x1000-100000
Resolution power= 0.2 nm
Tissue Preparation
F
*Preservation of the tissue.
Harden tissue-----> easy cut.
Prevents autolysis & putrifaction.
Preserve molecules.
Help staining
D
Gradual Dehydration
C
Removal of alcohol
I
Soft molten paraffin
*50 C xylol evaporates
*tissue filled with parrafin
e
Embedding
*Hard paraffin
*55C
S
M
S
Hx
E
Basic
Acidic
Acidic
Alkaline
Trichrome
Silver
Orcein
Elastic fibres--------->Brown
Reticular fibres--------->black
Neutral Stains
Leishmann stain for blood film
Vital Stain
Supravital Stain
Magnification in LM= up to 1500.
Resolution power= 0.2 um.
1mm=1000 um.
1um=1000 nm.
1nm=10 A.
Trypan blue
India ink
For collagen fibres
Brilliant crezyl blue
Reticulocytes in blood
Meta-chromatic stain
Trypan blue
Toluidine blue
Blue--------------> purple
Histochemical Stains
Glycogen
PAS
Immuno-histochemical stains
magenta red
Sudan III/Black
Fat
black
III
Enzymes
www.histologyonline.org
Full transcript