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Plant Pigments & Photosynthesis

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on 18 February 2014

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Transcript of Plant Pigments & Photosynthesis

Plant Pigments & Photosynthesis
Materials
Methods:
Data ( Light)
Data (Dark)
Introduction
Purpose: Use paper chromatography to separate the pigments found in spinach leaves.
By: Orpha Amazan, Miranda, Alex, & Meosha
In photosynthesis, plant cells convert light energy into chemical energy is stored in sugars and other organic compounds. The primary photosynthetic pigment in chloroplasts is chlorophyll. When testing the light reactions of photosynthesis, light energy excites electrons in plant pigments such as chlorophyll. Chromatography is a technique used to separate a mixture into its component molecules.
Hypothesis:
Variables
Amount of bicarbonate (baking soda)
The distance of the light
Amount of liquid solution
Amount of time under the light
Amount of time in the dark
Conclusion
Minutes
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Water and soap
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30cm CO2
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Minutes
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Co2 Dark
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50cm CO2 light
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Activtiy A:
Chromatography paper, green leaf, quarter, paper clip, and a ruler
Activity B:
Soap, Lamp, Four cups, Perminant marker, Leaf lets, dropping pipet, timer, and a calculator.
Activity A:

First we took a spinach leaf, and a quarter and ground the leaf 1.5cm from the bottom of the piece of chromatography paper, we then placed the piece of chromatography paper inside of a cup containing a solvent (Alcohol), the paper itself had to be held in the liquid with only a small portion being submerged in the solvent, so we made use of a paper clip to hold it in the liquid, this backfired, and ended up holding the paper at an awkward angle within the solvent, submerging one side more than the other.
Activity B:

First we punched holes within spinach leaves, then we made up four bicarbonate solutions, next we set up a lamp 30cm from the tabletop. The next step was to place leaf chads in a syringe, eject the air, then fill it with some of the bicarbonate solution, creating a vacuum by placing our thumb over top of the syringe. After the leaf chads sank, we then took them from the syringe, and placed into the bicarbonate solutions according to what was specified in the experiment.
Activity A: The solvent has separated the pigments into three different pigments, each varying in length, overall the the chromatography paper only had four pigments in total, including the starting green pigment, we theorize that if we had more time, we would have more lines of pigments.

Activity B: The results of the Photosynthesis lab went to prove that the concentration of light had an adverse effect on the rate in which photosynthesis took place, meaning that the closer the light was to the cup, the less effective it was in speeding up the rate of Photosynthesis. We theorize that if we were to redo this lab with all the required materials, and more time, we would have more accurate results.
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