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1. Dissolve a portion of
in 2.0 mL of water
2. Add 1.0 mL of
0.1% Ninhydrin Reagent
Mix thoroughly and heat in a boiling water bath for 2 minutes.
3. Note the color of the resulting solution. Record your observations.
Purpose and Principle
Ninhydrin test detects ammonia as well as
primary and secondary amines.
To know the principle and purpose of Ninhydrin Test
To know the purpose of the reagent
To show the reaction involved in this test and its positive result
To know the compound & groups of lipids involved in the test
To account for the precaution with Ninhydrin Reagent
Some lipids found in the brain like
have amide bonds which are easily cleaved by specific enzymes, resulting in compounds (like sphingosine and sphinganine) which have primary amine groups and will therefore produce a positive Ninhydrin test result.
Ninhydrin reagent shows up lipids containing amino groups.
It turns deep blue or purple color in the presence of free amino acids.
Group of lipids identified:
Non Glyceride Lipids
, are a class of lipids contain a backbone of sphingoid bases, a set of aliphatic amino alcohols that includes
. They were discovered in brain extracts in the 1870s and were named for the mythological Sphinx because of their enigmatic nature.
Precaution with Ninhydrin reagent
It can stain the skin because proteins in the body have an
N-terminus (ending where you have an amine) and it can react with those to evolve that deep bluish-violet color.
which are located throughout the body, but are partially important in nerve cell membrane. Its abundant in the myelin sheath that surrounds & insulate cells of the Central Nervous System.
Absence = Mutiple Sclerosis