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What is Unknown B #17

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nicole price

on 23 April 2014

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Transcript of What is Unknown B #17

What is Unknown B #17
Vouges-Proskauer Test (VP)
Positive is indicated by red
Gram Staining
Important differential test that tells about the organisms cell wall type
-gram negative will be pink
-gram positive will be purple
normally the first test ran that can tell about the cells morphology, size and arrangement

eye infection
wound infection
caused pneumonia
Conclusion
Organism was identified as
Serratia marcescens
flowchart
Serratia marcescens
History
-Chromobacterium prodigiosum


What can it really do?
Causes infections including...
-Urinary tract infection (UTI)
-Eye infections
-Wound infections
-Meningitis
-Upper respiratory infection

TESTS!
works cited
Unknown "B" flow chart
Gram Negative*
Alcaligenes, Aquaspirillum, Chromobacterium, Citrobacter, Cyclobacterium, Enterobacter, Escherichia, Francisella, Klebsiella, Kingella, Providencia, Pseudomonas, Salmonella, Serratia, Shigella
Falkinner, F.R., A. Hejazi.
Serratia marcescens
, April 1997. Journal of Medical Microbiology. vol. 46 no. 11. pg 903-912. April 2014. online.

Krige, Noel R., et al. Bergey's Manual of Derterminative Bacteriology ed. 9th. 1997. Lippincott Williams and Wilkins 2014. manual

Leboffe, Michael J., Burton E. Pierce. Microbiology Laboratory Theory and Application. ed. 3. 2012. Morton Publishing Company. 2014. manual

Newsom. S. W. B. Serratia marcescens: A colourful microbe.
January 2008 Journal of Infection Prevention vol. 9. no. 1. pg 25-27. April 2014. online.

Serratia
Bacteria
grimesii
liquefaciens
marcescens
pigment color
red
yes
no
Serratia grimmesii
Serratia liquefaciens
-copper color false positive

Unknown "A" flow chart
gram positive*
Bacillus, Cellulomanas, Corynebacterium, Enterococcus, Micrococcus, Mycobacterium, Staphylococcus, Streptococcus
Rods*
Bacillus, Cellulomonas, Corynebacterium, Mycobacterium
Cocci
Enterococcus, Sarcina, Staphylocccus, Streptococcus
Motile*
Bacillus, Cellulomonas
Non-Motile
Corynebacterium, Mycobacterium
Catalase positive
Staphylococcus
Catalase Negative
Enterococcus, Sarcina, Streptococcus
Endospore*
Positive
Bacillus
(circulans, megaterium
subtilis, cereus, coagulans,
licheniformis, polymyxa)
Endospore
Negative
Cellulomonas
Aerobic
Mycobacterium
Facultative
Anaerobic
Corynebacterium
Facultative
Anaerobic
Streptococcus,
Enterococcus
Aerobic
Sarcina
No Growth at
45 degrees C
Streptococcus
Growth at
45 degrees C
Enterococcus
VP Negative
circulans,
Megaterium
VP Positive*
subtilis, cereus,polymyxa
coagulans, licheniformis,

Citrate Negative
polymyxa
Citrate Positive*
subtilis, cereus,
coagulans, licheniformis
Anaerobic Growth
subtilis
Strict Aerobe
cereus, coagalans,
licheniformis
*
Horseshoe
Cyclobacterium
Rods*
Alcaligenes, Chromobacterium, Citrobacter, Enterobacter, Escherichia, Francisella,
Klebsiella, Kingella, Providencia, Pseudomonas,
Salmonella, Serratia, Shigella
Helical
Aquaspirillum
Motile*
Alcaligenes, Chromobacterium, Citrobacter, Enterobacter, Escherichia, Providencia, Pseudomonas,
Salmonella, Serratia
Non-Motile
Francisella, Kingella
Klebsiella, Shigella
Oxidase Negative
Francisella,
Klebsiella, Shigella
Oxidase Positive
Kingella

Aerobe
Francisella
Anaerobe
Klebsiella, Shigella
Urease Negative
Shigella
Urease Positive
Klebsiella
Aerobe
Alcaligenes, Pseudomonas

Growth on Mammals
Pseudomonas
No Mammal Growth
Alcaligenes
Faculatative Anaerobe*
Chromobacterium, Citrobacter, Enterobacter, Escherichia, Providencia,
Salmonella, Serratia
VP Positive*
Enterobacter,Serratia
VP Negative
Chromobacterium, Citrobacter, Escherichia, Providencia,
Salmonella
Decarboxylation*
Lysine Positive
Serratia
Decarboxylation
Lysine Negative
Enterobacter
Oxidase Positive
Chromobacterium
Oxidase Negative
Citrobacter, Escherichia, Providencia, Salmonella
Citrate Negative
Escherichia
Citrate Positive
Citrobacter, Providencia, Salmonella
Indole Negative
Providencia
Indole Positive
Citrobacter, Salmonella
Decarboxylation
Lysine Negative
Citrobacter
Decarboxylation
Lysine Positive
Salmonella
Methods (After preparation and incubation)
1)two loops of incubated broth into MRVP
2)add 15 drops of reagent A
3)add 5 drops of reagent B
4)read results every 10 minutes for results
Decarboxylation Lysine Test
Positive results are pink/purple
-orange and yellow are negative
Methods
1)inoculate the medium with the unknown
2)2-3 mm of sterile mineral oil on top
3)incubate for 48 hours
4)read the color results
Motile test
Positive results are indicated by fusion thru medium
-no growth away from stab is negative
Methods
1)inoculate with a needle
2)incubate for 48 hours
3)read the results
Methods
1)smear the sample onto the slide over a droplet of water
2)heat fix the slide allow to cool
3)cover the slide with crystal violet for 1 minute
4)rise with distilled water
5)cover with grams iodine
6)rise with distilled water
7)rise with 95% ethanol until runoff is clear
8)counterstain with safranin for 1 minute
9)rise with distilled water
10)read the slide under a microscope
Simple Staining
used as a measure to observe the morphology of the organism to highlight the features and organelles. More information is gathered from gram staining.
commonly known as a hospital acquired infection
originally viewed as a non-pathogenic water bacteria
was used as a biological marker for its red color
mistaken for fresh blood when growing on food
The bleeding bread
-religion
-war in Greece
-Witchcraft
Gaughren 1929
Nicole Price
Micro 2132 Sec 402
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