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The Effect of Sun Exposure on Stomatal Density

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bob joe

on 15 April 2014

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Transcript of The Effect of Sun Exposure on Stomatal Density

Stomatal Density in leaves exposed to Sun vs Those in the shade
P -Value: 0.00318 Alpha value: 0.01

Supports our hypothesis that leaves grown in sunny environment have a higher stomatal density

Higher rate of photosynthesis

Leaves exposed to sunlight undergo photosynthesis

CO2 is used in photosynthesis and enters plant through stomata
1. Collect 3 leaves from the shaded part of Antennaria plantaginifolia and 3 from the top sunny part.

2. Clean all the leaves making sure to remove trichomes.

3. Apply nail polish on the sunny sample leaves and let them dry.

4. Stick a piece of tape on the leaf and gently lift the tape off with the polish so an impression is formed.
Under 100x magnification, we found leaves exposed to sunlight had a higher stomatal density than those in shade

Alternative Hypothesis: Leaves grown in a sunny environment will have a higher stomatal density than leaves exposed to shade

Null Hypothesis: There is no difference in stomatal density among leaves grown in a sunny environment vs leaves grown in a shaded enviornment
5. Tape each of the impressions to clean slides and cut off excess tape

6. View impression under 100x magnification and find stomata

7. Count the stomata using a counter, atleast 3 times for that area
8. Average the count and convert it
to density using stomata per mm

9. Record data and repeat steps 6-8 with the impressions from the sunny leaves

10. Repeat steps 3-9 with the shaded leaves
Analyzing the Data
Switched magnification to 100x for better results

Multiplied area by 16

Used a T-test to analyze our results

Antennaria plantaginifolia
Extreme Heat
Need for water retention is greater than need for maximum photosynthesis

Stomatal density would not correlate directly with amount of sunlight
Temperate Zone
Utilize stomata more for photosynthesis than water retention

Leaves in shade don't need as much evaporative cooling
lower rate of photosynthesis
All food directly or indirectly come from plants
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