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MLST Database S.aureus

by Ruqaya Salhab
by

Ruqaya Salhab

on 8 December 2015

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Transcript of MLST Database S.aureus

Staphylococcus aureus database
Home page
MLST Home page
- MLST is a nucleotide sequence based approach for the unambiguous characterization of isolates of bacteria and other organisms via the internet.

-The aim of MLST is to provide a portable, accurate, and highly discriminating typing system that can be used for most bacteria and some other organisms. It is envisaged that this approach will be particularly helpful for the typing of bacterial pathogens.

-To achieve this aim we have taken the proven concepts of multilocus enzyme electrophoresis (MLEE) and have adapted them so that alleles at each locus are defined directly, by nucleotide sequencing, rather than indirectly from the electrophoretic moblity of their gene products.

MLST Map
when you click on any country you know the number of isolation in it.
Home page
Locus Query
give you the allel number
Database Query
to make research more specific.
Staphylococcus aureus database
Other tools
MLST
Database
S.AURUES

Multi Locus Sequence Typing
(MLST)
- MLST was developed in the laboratories of
Martin Maiden, Dominique Caugant, Ian Feavers, Mark Achtman and Brian Spratt.

- the site is hosted at
Imperial College
with funding from the
Wellcome Trust.


house-keeping genes.
Staphylococcus aureus DNA extraction protocol.
Lysis solution.
PCR conditions.
Introduction

Acknowledging use of the MLST database in your publications.

Obtaining an allelic profile and comparing your strains with those in the database

The seven loci and the primers and conditions used for PCR

ST & Profile Query
eBURST
Download data
Phylogenetic tree
Done by : Ruqaya M.Salhab
Bioinformatics
Palestine Polytechnic University
College of Applied Sciences
Applied Biology
supervisor : Miss. Abla G.Jaber
The End
Staphylococcus aureus shape

Staphylococcus
is group of bacteria that can cause a multitude of diseases.

for example :
skin infections :
such as pimples, impetigo, boils, cellulitis, folliculitis, carbuncles, scalded skin syndrome, and abscesses.
life-threatening diseases :
such as pneumonia, meningitis, osteomyelitis, endocarditis, toxic shock syndrome, bacteremia, and sepsis.
used to simplifies the problem of depicting the evolutionary relationships among closely related genotypes.
Full transcript