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DNA Extraction Workshop

Singularity U Executive Program March 2013
by

Raymond McCauley

on 3 July 2013

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Transcript of DNA Extraction Workshop

DNA Extraction Workshop
Clone Starter Kit
Teddy Fagin
Joyce Haven
Tomasz Kolinko
Geoff Ivison
Kristina Hathaway
Genetic Engineering 101 Class
Sunday, 9 am
biocurious0313.eventbrite.com
Your spit is full of cheek cells. Rub your cheeks to get more cells.
Need
some
help?
The SDS detergent in the soap
lyses
the cells.
They split open, spill their contents (including DNA) into the tube
DNA is negatively charged,
so it is repelled from other DNA.
To get it to bunch up,
we add the positive ions in salt
to
neutralize
the negative charge
The solution contains
proteases
that break down proteins.
This gets rid of some of the proteins
that were in your broken down cells.
Like pouring a layered shot...
Genetic Testing
Recommendations
Should I get a gene scan?
Should I get sequenced?
What about privacy?
How do I share with my doctor?
Probably not today...
Wait a bit (until 2014?)
2008:
2009:
2011:
2012:
2013:
2014:

2020:
$50,000
$10,000
$5,000
$2,000
$400
<$100

free with
Unless:
Rare disease
Cancer (tumor sequencing)
My personal opinion...
YES
Actionable information
scarce now
drugs are the exception
more on it's way
Genetic
Information
Nondiscrimination
Act
GINA
in United States, as of May 21, 2009
Limits health insurers use of genetic information
What does GINA not affect?
Life, disability, or long-term-care insurance
Some small businesses, in some ways
Federal employees and military
source: www.geneticfairness.org
Bring printed summary
Limit scope
Ask for "Shadow Record"
And...
Still need to find someone
(or some company)
to help you interpret
all those As, Cs, Gs, Ts...
US and World
First step for:
DNA fingerprinting, genotyping,
sequencing,
gene therapy...

4
Easy
Steps
Full transcript