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The Effect of Hypertonic and Hypotonic solutions on a Egg

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Bethany Cooper

on 11 October 2012

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Transcript of The Effect of Hypertonic and Hypotonic solutions on a Egg

The Effect of Hypertonic and Hypotonic solutions on an Egg By Claire, Justin, Bethany, and Alex Problem: How can we observe osmosis across a semi-permeable membrane? Hypothesis:
If we put the egg in 95% corn syrup and 5% water then it will be a hypertonic solution, meaning the egg will shrink because the water will be leaving the egg.

If we put the egg in 100% water then it will be hypotonic solution, meaning the egg will increase in mass because the water will be entering the egg. Materials:
1 chicken egg
solution 1: corn syrup
1 glass jar/cup
solution 2: tap water
string/yarn and ruler Experimental Procedure:
Independent Variable- Hypertonic and Hypotonic solutions
Dependent Variable- Mass of egg
Control group: Mass of egg before placed in solution
Constants: Equipment, egg
Experimental Group- Syrup egg, water egg

1. Rinse the deshelled egg and measure its mass in grams on a balance
2. Immerse the egg in 150 mL of corn syrup and, after one day, rinse and measure the mass of the egg
3. Record
4. Immerse the egg in 250 mL of tap water and, after two days, rinse and measure the mass of the egg
5. Record Analysis Questions:
1. The independent variable was the egg's condition and the dependent variable was the egg's mass.
2. Our control was the mass of a reuglar, deshelled egg, which we used to compared with the other egg conditions.
3. When the egg was placed in a water solution, the solution became hypertonic with water, and since there was more water than other organic molecules, the egg had undergone osmosis, causing the egg to receive most of the water and swell.
4. When the egg was placed in a syrup solution, the solution became hypnotic with water, and since there was more organic molecules besides water, the egg had undergone diffusion, causing the egg to receive most of the molecules and shrink.
5. After the egg was placed in 150ml of corn syrup, its moss decreased from 82.44g to 61.21g. After the new egg was placed in 250ml of tap water, its mass increased to 87.2g, more than its original mass. This data supported both of our hypothesis.
6. Because osmosis, the diffusion of water through a membrane in the opposite direction of the rest of the molecular structures, does not require enery, it is therefore a passive transport. Water is what is being moved and the direction it goes in is from high concentration to low concentration.
7. Passive and active transport are both forms of molecular transport, but they have different properties. A unique characteristic about acitve transport which gives it its terminology and name is that it requires chemical energy that is only found in the phosphate groups of an adenosine triphosphate molecule; passive transport only depends on the interaction of the molecules themselves that are involved. The carrier proteins found in each transport also are different in shape and maximum capacity: a glucose carrier protein in passive transport can only transport each molecule individually by passing it through its central pathway whereas a sodium-potassium pump has two openings that function with the help of ATP: the pump can carry up to three sodium and two potassium ions in their appropriate slots. Different molecules also require differnet transports as seen in the previous example.
8. The two solutions experienced diffusion over differnet lengths of time; potential errors could include destruction of the egg, changes in enviromental condition, or egg conditions (temperature, pH, other molecules), additon of different solutions, etc.
9. According to the data table, 250 ml of corn syrup and 150 ml of tap water were used in each solution. According to the bar graph, the masses of the normal egg (82.44g) and the water egg (87.2g) are progressively larger than the mass of the syrup egg (61.21g) , but the water egg's mass was a tad larger than the normal egg's mass. Data Table:

In conclusion, the data we recieved did support both of our hypothesis' which predicted that the egg would shrink when placed in the corn syrup solution(hypertonic) and would swell when placed in the water solution(hypotonic). The egg started out as 82.44 grams and after being placed in the corn syrup for a day came to the mass of 61.21 grams. The egg increased to 87.2 grams after being placed in the water for two days. Sources of Error:
Our group had the egg in the corn syrup for one day and then had the egg in the water for two days. The data would have been more accurate if the amount of time had remained constant.
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