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Knotweed Thesis

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Krystal Kappeler

on 1 May 2013

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Transcript of Knotweed Thesis

Krystal Kappeler
Dr. Sarah Ward
Dr. Cini Brown Knotweed Investigating the Spread of Invasive Knotweed
In the Rocky Mountain Region Abstract
Deliverables
Literature Review
Plant Profile: Fort Collins 1
Methodology
Results
Discussion
Future Research Introduction Krystal Kappeler
Dr. Sarah Ward
Dr. Cini Brown Background Nineteenth Century Garden Ornamental
Origination
Invasion Throughout Europe and US
Means of Spread
Colorado Department of Agriculture
Primers Purpose
Identify taxonomy across the Rocky Mountain Region
Master lab procedures and optimize results
Impact knowledge of hybridized weed invasions

Deliverables
Research Poster
Knotweed Website
Formal Research Paper

Components
Presentations
Publication
Public Site Deliverables
Common invasive species – Bailey 2007
Fallopia japonica
Fallopia sachalinensis
Fallopia baldschuanica
United States afflicted regions – Gammon & Grimsby 2007
Northwest and Northeast
Hybridization
United Kingdom Case – Hollingsworth & Bailey 2010
Invasion reduces diversity in native riparian plant communities - Aguilera 2010
U.S. Knotweed invasion didn’t cover the Rocky Mountain Region - Grimsby and Kesseli Literature Review Guard
Cells Fallopia japonica var. japonica Gender:
Female GPS Location: N40.587803, W105.092574, 5039 ft elevation, EW alley N of Mountain between Washington & Mac Plant Profile: Fort Collins 1 Petiole & Leaf Shape Morphology
Leaf Peels
DNA Extraction
Primer Dilution
Inter-Simple Sequence Repeats
PCR
Amplification
Gels and Staining Methodology Results
Primer
(GAG AGA GAG AGA GAG AYC)
Sex Determination
Leaf Hair and Shape
Taxonomic Identification
Educate People
Waste, Greenhouse, Alleyways Means of Spread
Reproductively and Clonally
Montana Plant Proximity
Will It Get Worse?
Hybridization
Oregon, Washington, etc.
Lack of Knowledge and Adequate Control
Enforcement of Invasive Species Discussion Genetic Comparison of All Plants
Temperature Tests
Growth-Limiting Factors
Determines Areas of Concern
What conditions limit invasion?
Methods of Control
Chemical, Environmental, & Bio Future Research Questions? Questions? Aguilera, A.A., P. Alpert, J.S. Dukes and R. Harrington. 2010. Bailey, J. 2007. Impacts of the invasive plant Fallopia japonica (Houtt.) on plant communities and ecosystem processes. Biol. Invasions 12:1243-1252.
Bailey, J.P., K. Bimova, and B. Mandak. 2009. Asexual spread vs. sexual reproduction and evolution in Japanese knotweed s.l. sets the stage for the “Battle of the Clones”. Biol. Invasions 11:1189-1203.
Gammon, M.A., E. Baack, J. F. Orth and R. Kesseli. 2010. Viability, growth and fertility of knotweed cytotypes in North America. Inv. Plant Sci. and Management 3:208-218.
Gammon, M.A., J.L. Grimsby, D. Tsirelson and R. Kesseli. 2007. Molecular and morphological evidence reveals introgression in swarms of the invasive taxa Fallopia japonica,F.sachalinensis, andF.bohemica(Polygonaceae) in the United States. Am. J. Bot. 94:948-956.
Grimsby, J. L., and R. Kesseli. 2010. Genetic composition of invasive Japanese knotweed s.l. in the United States. Biol. Invasions 12: 1943-1946.
Grimsby, J. L., D. Tsirelson, M.A. Gammon and R. Kesseli. 2007. Genetic diversity and clonal vs. sexual reproduction In Fallopia spp. (Polygonaceae). Am. J. Bot. 94: 957-964.
Hollingsworth M.L. and J.P. Bailey. 2000. Evidence for massive clonal growth in the invasive Fallopia japonica (Japanese knotweed). Bot. J. Linn. Soc. 133:463–472
Plant Industry Division. 2010. Colorado State - Listed Noxious Weeds. Invasive and Noxious Weeds. United States Department of Agriculture. http://plants.usda.gov/java/noxious?rpt&statefips=08. Accessed 4 Oct. 2010. Works Cited Website Results Table ? = non-blooming samples
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