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Visual lab briefing_Lab4_Osmosis

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by

Istvan Csatari

on 21 October 2016

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Transcript of Visual lab briefing_Lab4_Osmosis

Elodea canadensis
OSMOSIS
The observation of osmosis in the cells of
Elodea canadensis
(Canadian pondweed) and
Rhoeo discolor
(Boat lily)

Homework
You should not forget to answer the following questions...
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SEMI PERMEABLE
MEMBRANE
H
2
O
HIGH
Concentration
LOW
Concentration
OSMOSIS
solvent
solute
SOLUTION
ISOTONIC solution
Isotonic solution
HYPOTONIC solution
HYPERTONIC solution
Net water gain (+)
No net loss or gain
Net water loss (-)
Cell swells
Cell shrinks
The cell is happy
PLASMOLYSIS
Cell is dehydrated
LYSIS
Cell is over-hydrated
EQUILIBRIUM
Microscopic observations
Elodea canadensis
(Canadian pondweed)
Rhoeo disolor
(Boat lily)
SAMPLE
a piece of a leaf
SAMPLE
lower epidermis
No treatment
WET MOUNT!
10 % NaCl
Plasmolysis
WATER
Deplasmolysis
or
20 % sucrose
Elodea canadensis
No treatment
WET MOUNT!
10 % NaCl
Plasmolysis
WATER
Deplasmolysis
20 % sucrose
Rhoe discolor
Did
Elodea
and
Rhoeo
cells change shape? Why or why not?
What is the difference between a hypertonic solution and a hypotonic solution?

What will happen to plant cells that are placed in a hypertonic solution?
Compare the location of chloroplasts in normal (turgid) and plasmolyzed (flaccid) cells you have observed.
What was the cause for the change in the chloroplast location in the different solutions?
Full transcript