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Jowana alawie

on 18 July 2017

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Exploring Solid Phase Peptide Synthesis
Winter Scholarship Project - July2017
New Concepts

Lab Safety in Depth
New Concepts (II)



Synthesize an updated version of the GLP-1
peptide in order to prevent rapid excretion
through the kidneys and thereby extend
its half-life.
What did I Encounter?
New
Techniques
Lab Safety
in depth
New
Equipment
Microwave Irradiation
The use of
direct microwave heating is reported to reduce the chemical
reaction time, reduce side reactions, increase yields and
improve reproducibility.
New Concepts
Lab safety ;
- Inductions
- Training Modules
- Tour
- Risk Assessment

- General Workplace
Safety Training
- Annual Fire Safety
Training
- Code of Conduct
Training
- EO Online Equity and
Diversity Training



http://www.uq.edu.au/ohs/
Lab Coat
Goggles
Enclosed shoes
Fume cupboard
Coupling Reagents :
OxymaPure/DIC, HBTU/DIPEA

Protecting groups :
Fmoc , Boc, IvDde

Resin:
Rink amide Resin

Linker:

Protein to be synthesized and modified:
GLP-1

Test Cleavage

Ninhydrin test
Blue Solution - Amines present = FAIL

Yellow solution- amines not present = PASS; protein is protected


Your best friend - The synthesizer
HPLC
Centrifuge
Desiccator
Spectrophotometer
Freeze Drier
FAIL
PASS
The first N-protected amino acid is loaded
onto the resin
After removal of the protecting group, the second N-protected
amino acid is coupled to the first
This sequence is
repeated until all desired amino acids are loaded
The last step is the cleavage of
the final peptide
from the polymer support.
Solid Phase Peptide Synthesis
* Coupling
Reagents
*TFA
My greatest appreciation goes to;

Dr. Peter Moyle
Ebrahim Alavi


For their time and effort enriching me with new ideas and concepts.

Jowana alawie

Full transcript