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Winter Scholarship Project - July2017
Lab Safety in Depth
New Concepts (II)
Synthesize an updated version of the GLP-1
peptide in order to prevent rapid excretion
through the kidneys and thereby extend
What did I Encounter?
The use of
direct microwave heating is reported to reduce the chemical
reaction time, reduce side reactions, increase yields and
Lab safety ;
- Training Modules
- Risk Assessment
- General Workplace
- Annual Fire Safety
- Code of Conduct
- EO Online Equity and
Coupling Reagents :
Protecting groups :
Fmoc , Boc, IvDde
Rink amide Resin
Protein to be synthesized and modified:
Blue Solution - Amines present = FAIL
Yellow solution- amines not present = PASS; protein is protected
Your best friend - The synthesizer
The first N-protected amino acid is loaded
onto the resin
After removal of the protecting group, the second N-protected
amino acid is coupled to the first
This sequence is
repeated until all desired amino acids are loaded
The last step is the cleavage of
the final peptide
from the polymer support.
Solid Phase Peptide Synthesis
My greatest appreciation goes to;
Dr. Peter Moyle
For their time and effort enriching me with new ideas and concepts.