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Science Fair Project

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Erica Garland

on 4 April 2013

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Transcript of Science Fair Project

What Type of Makeup Grows the Most Bacteria? By: Erica Garland My hypothesis was that the mascara would grow the most bacterial colonies. The results indicated that this hypothesis should be considered correct because out of all the cosmetics tested, the mascara had the most bacterial growth, most likely due to it being the ideal living environment for bacteria. Because of the results of the experiment results, I am a bit more hesitant when applying multiple layers of mascara to my eyelashes now that I am aware of the bacteria that thrives in mascara bottles and wands. If I were to conduct this science fair experiment again, I would test more cosmetic products, and see if I can obtain a microscope in order to get a closer look at the bacterial colonies. Question Out of the top five, most commonly used cosmetic products, which will grow the most bacteria? Hypothesis Conclusion Results of Experiment Materials Background Research I hypothesize that when mascara, blush, liquid foundation, lipstick, and pencil eyeliner are tested for bacteria, the mascara will grow the most bacteria and the blush will grow the least. I based this hypothesis off the fact that mascara bottles are warm, dark environments that frequently receive pumps of oxygen along with containing ideal PH levels for bacteria. Blush, however, has the exact opposite living conditions as mascara. Procedure Sterilize the four plastic petri dishes by using the bleach, anti bacterial soap, and rubbing alcohol, then place them in a sterile environment to dry. Take the materials you will need into the sterile area and apply a pair of latex gloves to your hands. Fill the sterilized petri dishes with agar jelly and allow the agar jelly to dry. Using a cotton swab, take a sample of one of the cosmetic products and apply it to the agar jelly in one of the petri dishes. Close the lid of the petri dish immediately after applying the agar jelly with the makeup, and label the lid with the type of makeup. Dispose of the latex gloves and apply a new pair. Then repeat the last two steps with the other four types of makeup. Once this has been completed with all five cosmetic products, place the petri dishes in a warm, sterile area. Wait twenty four hours, then record the amount of bacterial colonies. Rubbing alcohol Four plastic petri dishes Agar jelly Blush Liquid foundation Mascara Lipstick Eyeliner pencil Non-abrasive cloth Anti-bacterial soap A box of disposable latex gloves At least 4 cotton swabs Bleach A sterile environment Rubbing alcohol Paper Pen/pencil Variables Controlled Variable Independent Variable The makeup being tested for bacterial growth. Dependent Variable The amount of bacterial growth in each petri dish. The petri dish without makeup. Experimental Data Averages In my research, I focused mainly on the necessities bacteria must have in order to grow in an environment. In my research, I found that the ideal growing environment of bacteria is typically dark, has a source of moisture, nutrients, and oxygen, and has a PH level of around 7. Out of the cosmetic products that I am testing, the one that would match this "environment" the best would be the bottle of mascara, due to the PH level of 7, high levels of moisture, sufficient supply of oxygen, source of nutrients, warm temperature, dark pigmentation. In my research, I also decided to look into bacterial growth methods that would give me the most accurate results. Several websites and books regarding bacterial growth stated that plastic petri dishes are best for experiments, and all instruments used in the process must be kept as clean as possible, so outside bacteria does not affect the bacteria that is about to be cultivated. Lehman, Christine. "Where Do Bacteria Live?" EHow. Demand Media, 16 Nov. 2008. Web. 03 Apr. 2013. Bibliography Donald, Ashley. "Bacteria in the Boys' & Girls' Living Environments." Bacteria in the Boys' & Girls' Living Environments. N.p., 08 Oct. 2001. Web. 03 Apr. 2013.
E, Jenny. "Types of Bacteria in the Environment." EHow. Demand Media, 09 Sept. 2010. Web. 03 Apr. 2013.
"Top 10 Most Popular Cosmetic Products." Beauty 101 RSS. N.p., n.d. Web. 03 Apr. 2013
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