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RE East Zone Science Fair 2010 Presentation

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Yong Sheng Tay

on 13 August 2010

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Transcript of RE East Zone Science Fair 2010 Presentation

Lazarus Chok
Russell Ang Zen
Tay Yong Sheng
Timothy Tan
House dust mite as seen under a microscope Belongs to the genus Dermatophagoides Theoretical Background Found in every home:
Bed sheets
Pillow Casing
Stuffed toys Conditions for growth:
Steady supply of food: main diet - skin cells
Impact of Dust Mites on Health Produce droppings containing the protein DER p1
Many people are allergic to this protein
Increases severity of symptoms among asthma sufferers and sufferers of other respiratory problems
Main cause of allergic rhinitis
Common HDM control methods Cleaning and Sanitizing
Use of repellents
Natural Introduction Objectives Which type of plant-derived essential oil (EO) is most effective in repelling HDM?
Does the concentration of the EO affect its effectiveness in repelling HDM?
Impact of research Exposure to HDM linked to allergic rhinitis
Use of synthetic chemicals to control dust mites e.g. benzyl benzoate + tannic acid
Effect of synthetic chemical pesticides on environment and humans Residual chemicals that are toxic and carcinogenic
Focus on natural repellents
Alternative botanical 'pesticide' Safer, less toxic, more environmentally-friendly

Reduce reliance on synthetic chemical repellents Procedure Culturing/Breeding HDM
1.House dust sucked up from used mattress

2.1 cm3 of house dust spread onto a piece of cloth

3.Moisture added to cloth

4.Left to settle for 96 hours
Applying EO for experiment 1
1.EOs were mixed into 0.1% concentrations with water

2.For each cloth, 1ml of EO was sprayed onto it

3.Each set-up was repeated another two times

4.Control set-up created and repeated another two times

5.Left alone for 72 hours

15 pieces of cloth laid out on the towel for experiment 1 Cloths were sandwiched between two towels
Applying EO for experiment 2
1.Eucalyptus and lavender EOs were mixed into 0.05%, 0.1% and 0.5% concentrations with water

2.For each cloth, 1ml of EO was sprayed onto it

3.Each set-up was repeated another time

4.Left alone for 72 hours
Micropipettes were used to achieve high accuracy
Collecting results 1.Three pieces of adhesive tape were placed onto the fabric cloth

2.Placed on a hot plate for 3 minutes (50 Degrees Celsius)

3.HDM counted in a zigzag fashion
Zigzag movement when counting HDM
Results Lavender EO has the best dust mite repelling properties

Eucalyptus EO was the second most effective dust mite repellent
Results not in accordance with first hypothesis
As the concentration of EO decreased, the number of HDM counted increased
Our second hypothesis was proven correct
Observation Discussion 2 Properties Antimicrobial Antibacterial Keratinolytic bacteria Killed when antibacterial agents are applied Endosymbiotic, assist HDM in digesting keratin HDM can no longer digest keratin from dead skin cells as effectively
Loss of food source Relationship between HDM and fungi Removal of fungi with linalool Another food source lost Conclusion Natural EO mixture Commercially available Varied application methods This shouldn't be the end. Sadly, this is the end of our presentation.
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