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Transcript of Southern Blotting
How Does It Help?
Does It Work Well?
Khalsa, Guruatma. "Mama Ji's Molecular Kitchen." Southern Blotting | ASU - Ask A Biologist. Arizona State University, 12 Apr. 2010. Web. 13 Feb. 2017.
Young, Donald, and Debra Leonard. "Issues in Genetic Testing." Clinical Chemistry. American Association for Clinical Chemistry, June 1999. Web. 13 Feb. 2017.
"Introduction to Southern and Northern Blotting." Sigma-Aldrich. Sigma-Aldrich Co. LLC, n.d. Web. 13 Feb. 2017
Is There Controversy With Southern Blotting?
By Alyse Mize
What Is It?
How Old Is It?
How Does It Work?
1. Digest the Enzyme with an restriction enzyme.
2. Run the digestion an an agarose gel
3. Denature the D.N.A.
4. Transfer the denatured D.N.A. to the membrane
5. Probe the membrane with the labeled ssD.N.A.
6. Visualize you radioactively labeled D.N.A.