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Locating the Transgene on Drosphila Melanogaster Chromosomes

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Clay Rosinski

on 15 April 2014

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Transcript of Locating the Transgene on Drosphila Melanogaster Chromosomes

Cross A4
Parental Generation
F1 Generation
Introduction
F1 Cross
F1 Male Meiocyte Chromosomes
Locating the Chromosomal Position of the Transgene on
Drosophila Melanogaster

Presented by:
5'-Clayton Gordy, Mitchell Miller, Clayton Rosinski, Caitlyn Wickersham-3'
D. melanogaster have a pair of sex chromosomes (XY/XX) and three pairs of autosomal chromosomes numbered 2,3,4. The majority of their autosomal genes reside on chromosomes 2 and 3 (Leicht et al, 103).
We focused on traits for wing shape, sex, and bristle type in order to pinpoint possible locations of the transgene because each of these traits lie on separate chromosomes (excluding chromosome 4) and are easily identified.
The transgene causes the flies to have distinctive red eyes when it is expressed. The transgene is dominant to the mutagen white eye allele. The location of the transgene was limited to either the sex chromosomes, or autosomal chromosomes 2 and 3.
To actually determine which chromosome the transgene was located on, a specific cross was formed.
Female Parent
(Momma Fly)
Genotype:wP{w+}/wP{w+}, Cy+/Cy+,Sb+/Sb+
Phenotype: red eyed, wild type wings, wild type bristles
Male Parent
(Papa Fly)
Genotype:w/y,Cy+Xa/CyXa+, Sb+/Sb
Phenotype: white eyed, curly wings, stubble bristles
Offspring of momma and papa
F1 Male
F1 Female (Virgin)
Genotype: w/w,Cy+/Cy+,Sb+/Sb+
Phenotype: white eyed, wild type wings, wild type bristles
F1 Male Chromosomes
Transgene on the X chromosome
Transgene on chromosome 2
Transgene on chromosome 3
Potential Gametes with transgene on X
Potential Gametes with transgene on chromosome 3
Potential Gametes with transgene on chromosome 2
Transgene Location Predictions
If the transgene is located on the X chromosome, then all male offspring will have white eyes, and all female offspring will have red eyes.
If the transgene is located on chromosome 2, then flies with white eyes will have curly wings, and all flies with red eyes will have straight wings, regardless of gender.
If the transgene is located on chromosome 3, then flies with stubble bristles will always have white eyes, and flies will straight bristles will have red eyes, regardless of gender.
Results
Our Data


Pooled Data
Our Data
Chi-Square Assuming Transgene is Located on X
Chi-square=822.776
P value=incredibly small
Chi-Square Assuming Transgene is Located on Chromosome 2
Chi-square=1.124
P value=much greater than .25
Chi-Square Assuming that Transgene is Located on Chromosome 3
Chi-square=2.206
P value=much greater than .25
Conclusions
Based on Our Data...
X
3
2
Based on the Pooled Data...
3
2
X
The chi-squares of the three different hypotheses shows that the chance of the X chromosome and the transgene sorting independently is almost zero. The chi-squares give a large probability that the transgene does sort independently of both chromosome 2 and 3. Thus it is highly likely that the transgene is located on the X chromosome.
References
Leicht, Brenda & McAllister, Bryant. 2013. Foundations of Biology 1411. 2nd ed Sounthlake TX, Fountainhead Press. 203p
http://bsp.med.harvard.edu/node/105
http://pk-photography.blogspot.com/2010/08/drosophila-melanogaster-life-cycle.html
Acknowledgments
Genotype: wP{w+}/Y,Cy/Cy+,Sb/Sb+
Phenotype:red eyed, curly winged, stubble bristled, male
Genotype:w/Y,Cy/Cy+P{w+},Sb/Sb+
Phenotype:red eyed,curly winged, stubble bristled, male
Genotype:w/y,Cy/Cy+,Sb,Sb+P{w+}
+
Phenotype:red eyed, curly winged, stubble bristled male, male
Y,Cy+,Sb+
Y,Cy,Sb+
Y,Cy,Sb
Y,Cy+,Sb
wP{w+},Cy+,Sb+
wP{w+},Cy,Sb+
wP{w+},Cy,Sb
wP{w+},Cy+,Sb
Genotype
Genotype
Y,Cy+P{w+},Sb+
Y,Cy+P{w+},Sb
w,Cy+P{w+},Sb+
w,Cy+P{w+},Sb
w,Cy,Sb+
w,Cy,Sb
Y,Cy,Sb
Y,Cy,Sb+
Genotype
w,Cy+,Sb+P{w+}
w,Cy+,Sb
w,Cy,Sb+P{w+}
w,Cy,Sb
Y,Cy,Sb
Y,Cy+,Sb
Y,Cy+,Sb+P{w+}
Y,Cy,Sb+P{w+}
F1 Flies Selected
Genotype: wP{w+}/Y,Cy/Cy+,Sb/Sb+
Phenotype: red eyed, curly wings, stuble bristles
Genotype: wP{w+}/Y,Cy/Cy+,Sb/Sb+
Phenotype: red eyed, curly wings, stuble bristles
Title and Group Memebers Names: Clayton Gordy
Introduction: Caitlyn Wickersham
Description of Crosses: All members
Illustrations: Mitchell Miller
Predictions:Clayton Rosinski
Results:Clayton Gordy, Caitlyn Wickersham
Conclusion: Mitchell Miller, Clayton Rosinski
References:Caitlyn
Acknowledgments: Clayton Gordy
Master P(rezi): Clayton Rosinski
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