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Pericytes Laurea

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thiago tomazi

on 23 December 2013

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Transcript of Pericytes Laurea

What are they?
Determination by:

Literary Research
Markers! CD146 and NG2
Morphology describe on literature

Where can we find them?
Staining of Renal microscopical samples
for Cd146 and Ng2 + Controls

What can they do?
Differentiation capacity into fat, bone and cartilage

Immunomodulation - Immunosupression of CD3/8 activated T-Lymphocytes
Characterization of Renal Pericytes
The percentage of Pericytes in culture from crude kidney material is very low


The duplication time of Pericytes is slower then from the other cells from crude kidney material.


CD146 is highly expressed on cells expressing NG2 cells as NG2 is highly expressed on cells expressing CD146


The expression of CD146 on crude kidney material is highly variable, while the NG2 expression showed a much more homogeneous positive population.


The combined presence of both markers together with a corresponding morphology described in the literature suggests that the cells used by us in our test are Pericytes.

This cells can differentiate into Bone and Fat Tissue, this can be a source for future organ repair

There is evident signs of immunnosupression capability presented by this cells, that calls for further research as they could be uses as MSC in the protection of new transplants or in lowering autoimmune activity against the kidney
Conclusions
Inhibition of proliferation of CD3/8 activated T-lymphocytes essays
Cd146+ k4
Cd146+ k4
Control k4
Control k4
Cd 146 + NG2+ k8
Cd 146 + NG2+ k8
No selection
“non-pigmented adventitial cells” or "cells with ramified protoplasmic processes" Rouget 1873
adventitial cells,
Fibroblasts,
Rouget cells,
Pericytes ( Zimmerman in 1923)
"intramembranous pericapillary cells", (Uhar and Hartmann)
"pericytes” (1956 Maeda)
"pericapillary cells",
"perivascular cells",
extramural pericyte" (Cogan, Toussaint, and Kuwabara, 1961).
Mural cells (Cogan, Toussaint, and Kuwabara, 1961)
"undifferentiated cells", (Bernstein, 1961; Bloodworth, 1962; Missotten, 1962; Hogan and Feeney, 1963; Ishikawa, 1963;
Kuwabara and Cogan, 1963; and others);
"intramural pericyte“,(Fawcett, 1963)
Characterization of Renal Pericytes and its Multipotentiality


Thiago Tomazi





Gaetano La Manna, Paola Todeschini, Marten Engelse,
Cees van kooten, Ton J Rabelink
Kidney Department, Leids University Medical Center, Leiden, The Netherlands
Inhibition of proliferation of CD3/8 activated T-lymphocytes essays
Cd146+ k4
Cd146+ k4
Red- alfa-SMA - smooth muscle, fibroblasts


White – NG2 – Pericyte

Green – CD34 – Hematopoietic (T cells- infiltrate) ) vascular-associated tissue
Fotos zeiss 700
Confocal Images
Red- alfa-SMA - smooth muscle, fibroblasts


White – NG2 – Pericyte

Green – CD34 – Hematopoietic (T cells- infiltrate) ) vascular-associated tissue

Blue- Nuclei
Confocal Images
CD146+ NG2+ k4
CD146+ NG2+ k4
CD146- NG2+ k4
CD146- NG2+ k4
NG2 - k3
NG2 - k3
Acknowledgements
Crude kidney
Gaetano La Manna
Paola Todeschini
Ton Rabelink
Marten Engelse
Cees van koot
Full transcript