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The effect of colored light on plants

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on 11 February 2014

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Transcript of The effect of colored light on plants

The effect of

colored light on

Graph of data
Autotrophs and Heterotrophs
The difference between autotrophs and heterotrophs in the way that they obtain energy. Autotrophs obtain energy through nonliving sources such as sunlight or chemicals, and hey then convert these into energy in the form of glucose through processes such as photosynthesis and chemosynthesis. Heterotrophs on the other hand obtain energy by consuming other autotrophs and heterotrophs, and then using them for energy. Both of these organisms need processes like photosynthesis, and cellular respiration in order to survive. Photosynthesis is how a plant creates energy, and stores it, whereas cellular respiration is how a cell obtains biochemical energy produced by photosynthesis.
Plants are autotrophs, which means that they can provide nutrients for themselves using inorganic materials. They obtain nutrients through the process of photosynthesis, which uses light and carbon dioxide to provide nutrients.
On the leaves of plants, there are many different sections with different functions. Leaves use chloroplasts, which absorb light, to turn carbon dioxide and water into a simple sugar that is used for growth and maintaining a healthy state in the plant. Stomata release water and oxygen from the plant and also take carbon dioxide in.
Our research question
Our test was designed to record the affects that colored light had on the rate of photosynthesis measured by the oxygen production of 3 plants.
Experimental Hypothesis

If plants are exposed to different color light then they will undergo photosynthesis, producing oxygen at different rates because pigments in plants absorb different wavelengths, giving more or less energy for photosynthesis, causing plants to expel oxygen at different rates.

Null Hypothesis

If plants are exposed to colored light then they will under go photosynthesis, producing oxygen at the same rate because pigments absorb all light, making photosynthesis identical in the same plant when exposed to different colored light.

One variable in this experiment that may have had an effect on the results is photorespiration.
Photorespiration occurs when a plant fixes oxygen into the calvin cycle instead of Carbon. This results in the creation of a PGA molecule and a dual carbon molecule that the plant must turn into something useful
This is number 15 on the rubric
Variables (cont.)
Photorespiration usually occurs when there is a high oxygen concentration in the air and the rubisco in the calvin cycle cannot determine carbon from oxygen.
One way that we could improve the experiment is if we used a C4 or a CAM plant, because both C4 and CAM plants have a rubisco enzyme that can tell the difference between carbon and oxygen.
#15 continued
The independent variable in this experiment was the color of the light that we used.
The lights that we used were blue, green, and white. We chose different colored lights because plants absorb different wavelengths and it affects the rate of photosynthesis.
The dependent variable in this experiment is the amount of oxygen that is in the bottle. We chose to measure the amount of oxygen in the bottle because if photosynthesis works then the amount of oxygen will increase.
In conclusion, we did accept our experimental hypothesis, except the results were not what we expected.
We expected the white light to produce the most oxygen, the blue light to produce the second most, and the green light to produce the third most. What happened is the green light produced the most oxygen by far, and the blue and white lights actually lost oxygen.
One reason for this may be that the blue light and the white light performed photorespiration. One thing to also notice is that there were higher oxygen levels in the white and blue light plants at the start, and when there is a high oxygen concentration in the environment, the plants are more likely to perform photorespiration. The plants that we used were C3 plants, so they were prone to photorespiration. At the start of the experiment, the plant that was under white light had oxygen levels of 14.10%. The one under the blue light had levels of 16.22%, and the plant that was under the green light only had oxygen levels of 4.89%.
1 Vernier probe
1 Light, blue
1 Light, white
1 Light, green
30 mL of water, 10mL for each plant daily.
3 plants, same species.
3 plant holders.

Observation of Data
Our experiment didn't meet our predicted results. We believed that since pigments in plants absorb the blue wavelength of light more than any other, that this plant would produce the most oxygen as it would under go the most photosynthesis. The green light was predicted to produce the least amount of ocygen, as plants don't absorb very much green wavelength. Our white lightbulb was supposed to cause the plant to produce oxygen at a level that was in between that of blue and green lights. The opposite of our expected results happened, green having the most oxygen produced and blue having the least.
Proposed experiment
If we were to redo our experiment, we would put another probe to measure the carbon dioxide levels. This would ensure accurate data, because if the oxygen levels went up and carbon dioxide levels decreased it could be determined that the plant was using carbon dioxide for photosynthesis, thus producing oxygen. We would leave all of the other aspects of our experiment the same.
1. Add 10 ml of water to each plant.
2. Place plant B and and plant C in separate cabinets.
3. Place plant B with the blue light, and plant C with the green light.
4. Put plant A under a grow light.
5. Let each plant sit for 25 minutes.
6. Measure the wavelength absorption using the vernier probe.
7. Record results in a datatable.

Data Table/ Appendix
White Green Blue
Beggining: 14.1 4 .89 16.22
20 minutes: 13.31 8.73 15.41
40 minutes: 13.67 8.61 15.46
Difference -0.43 3.72 -0.76
Probe we used to measure oxygen levels in the plants to see how they differed


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The equation for photosynthesis is 6CO2+ 6H2O yields C6H12O6+ O2. This equation shows that photosynthesis takes in carbon dioxide, and water, and uses the process of photosynthesis to convert it into energy. The way that it works, is the sunlight forces electrons from water molecules into the carbon dioxide molecules forming a sugar. This oxidizes the water meaning that it loses electrons, and the carbon dioxide molecule gains them. This sugar is then stored in the form of chemical energy which will be obtained through cellular respiration.
Products of Photosynthesis
The beneficial products are the main purpose of photosynthesis. This would be the glucose produced, and the waste product produced during photosynthesis is oxygen gas, or O2. The plant doesn’t need this, so it will leave the plant through its stomatal openings. The glucose is moved to the xylem and phloem that will be able to move the nutrients from glucose to the rest of the plant. It is transported with the help of pressure from the obtainment of water.
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