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MOL 350 Independent Project
Transcript of MOL 350 Independent Project
cell cycle-regulated proteins Significantly downregulated by DNA damage Clb2 is a B-type cyclin involved in the cell cycle progression. Activates Cdc28p to promote the transition from G2 to M phase Goes down with methylmethane
sulfonate treatment (doses) Gene Expression Appear Correlated
According to Microarray Final Conclusion from Canavanine:
Suggests that the deletion phenotype is
similar for wildtype. The loss of the
allele does not impact mismatch repair. Our plate did not grow even for the wildtype and the experimental allele but grew in the null allele Proposed possible relationship with Msh2:
The activation of Cdc28 serves as a checkpoint for G2 to M phase transition. If Msh2 is activated, Ynl058c will be downregulated by the DNA damage (possibly through interaction with Msh2), and thus the cell will be unable to pass from G2 to M Msh6 strongly upregulates Clb2. It may be involved with the function of Msh6 in the post-mismatch recognition steps MSH2 and CLB2 are both activated by MSH6 FOA plate had traces of colonies for the null allele. Lack of large colonies attributed to the fact that it was not given sufficient time to grow Adipate shows no growth. It perhaps exhibits similar selective behavior to FOA and requires an extended period of time for colonies to appear. MASTER PLATE