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bio safety barriers
Transcript of bio safety barriers
Level 3 primary barriers
All manipulations of infectious materials are conducted in Class II or Class III biological safety cabinets.
When a procedure or process cannot be conducted within a biological safety cabinet, appropriate combinations of personal protective equipment, such as respirators and eye/face protection, are used. Respiratory and face protection are used when in rooms containing infected animals.
Level 2 secondary barriers
agents associated with human disease.
Facilities that house restricted agents must have lockable doors.
Biological safety cabinets must be installed to ensure operation within all applicable parameters.
An eye wash station should be readily available.
Level 1 primary barriers
Biological cabinets are generally not used with this level of agent.
Employees are encouraged to wear lab coats, gowns or uniforms to prevent contamination of street clothes.
Gloves should be worn if skin is broken or a rash is present.
Eye wear should be worn for procedures where splash of microorganisms or hazardous materials is anticipated.
Level 2 primary barriers
Biological safety cabinets, preferably Class II, should be used when potential for infectious splash or aerosols exist or high concentrations/large volumes of infectious agents are in use
Face protection, including goggles, masks, face shields or splatter guards, should be used for anticipated splashes or sprays for work that must be performed outside the biological safety cabinet.
Protective clothing is worn while in the lab and removed and left in the lab before leaving for non-lab areas. All protective clothing is disposed or laundered by the institution and should never go home with personnel
Gloves are worn when hands may contact infectious materials; double-gloving may be appropriate depending on the application procedure
Disposable gloves may not be reused or washed. Hands are washed upon removal of gloves.
Level 4 primary
All procedures within the facility are conducted in a Class III biological safety cabinet or in a Class II biological safety cabinet used in conjunction with one-piece positive pressure personnel suits ventilated by a life-support system
Level 3 secondary barriers
Agents with potential for respiratory transmission, may cause serious and potentially lethal infection.
Each lab contains a hands-free sink for handwashing located near the room exit door.
Walls, floors and ceilings must be constructed for easy cleaning and decontamination.
All windows must be closed and sealed.
The combined use of laboratory practices, laboratory facilities and safety equipment
Why use biosafety practices? To protect:
Level 1 secondary barriers
is the basic level of protection common to most research and clinical laboratories, and is appropriate for agents that are not known to cause disease in normal, healthy humans.
Labs should have doors to limit access.
Each lab needs a sink for handwashing.
Design of the lab should facilitate easy cleaning, without rugs or carpets.
Level 4 secondary
Dangerous and exotic agents that poses a high risk of life -threatening disease.
Agents (all viruses) include Marburg virus, Ebola virus.
Sahar Aljahdali, Afnan Gaied ,Hadeel Kalbona
Ghada Alsaied , Nahla Albeladi ,
Ghadeer Bin Rabbaa