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The Wonders of Capillary Electrophoresis
Transcript of The Wonders of Capillary Electrophoresis
Types of Electrophoresis
Capillary Zone Elecrophoresis
Capillary Isoelcectric Focusing
1800's - Electrophoresis is discovered and first practiced.
1967- Hjerten constructed the first fully automated capillary free zone electrophoresis apparatus
1970's Virtanen and Mikkers et al. reported on the use of 200 μm i.d. glass and Teflon capillaries
1981- the first high performance CE separations were achieved by Jorgenson and Lukacs
1989- first fully automated commercial capillary electrophoresis system by Beckman Coulter
Easy to Use
Short Analysis Times
Can Be Automated
Extremely Versatile (CZE, CGE, CIEF, ITP, EKC, MECC, MEEKC, NACE, CEC)
Simultaneous Separation of Anions and Cations
Small Sample Sizes
Very Little Chance of Cross-Contamination
Further Investigate Firearm/Gunshot Residue
-How it works
Short detection path
Detector portion must be bare
pH can affect the separation
If there is too much analyte, separation efficiency is reduced
Lack of data on reliability
Many different methods to get results
No standard retention times
We will gladly take questions at this time!
Disadvantages of Capillary Electrophoresis
Advantages of Capillary Electrophoresis
DNA Analyst Training. n.d.
Felhofer, Jessica L., Lucas Blanes, and Carlos D. Garcia. "Recent development in intstrumentation for capillary electrophoresis and microchip-capillary electrophoresis." Electrophoresis (2010): 2469-2486.
Kim, Jiwon. Capillary Electrophoresis. Charlotte, 2010. Web.
Larsen, Delmar. Capillary Electrophoresis. Berkeley: US Davis ChemWiki, 12 June 2012. web.
Lewis, S. W. Capillary Electrophoresis in Forensic Chemistry. 2013.
"NFSTC Service Serving Justice." 2010. nfstc.org. Web. 12 June 2014.
Pascali, Jennifer Paola, Federicia Bortolotti, and Franco Tagliaro. "Recent advances in the application of CE to forensic science, an update over years 2009-2011." Electrophoresis 2012: 117-126.
Michalke, B. Potential and limitations of capillary electrophoresis inductively coupled plasma mass spectrometry. J. Anal. At. Spectrom., 1999, 14, 1297-1302
Tilstone, Bill. "DNA Analyst Traning." 2010. nfstc.org. web. 12 June 2014.
How It Works
Inappropriate conditions can cause
Samples sticking to capillary walls
Peaks can become hard to read
Inconsistent retention times
An EC works by separating compounds through a medium when exposed to an electric charge
The Ions move through capillary during electrophoresis
The cations flow toward the negative terminal, and the anions flow to the positive terminal
The most common models used are the ABI 310 and the ABI 3100, and they are made of 3 main components:
2. Gel Block-
where the anode electrode is located and it provides a positive electrical current
3. Detection Center-
Where separation occurs
- Functions in holding sample trays and presents them to the capillary for injections
Demonstration of how Capillary Electrophoresis works
What is Electrophoresis?
Electrophoresis is an analytical method used in the laboratory that results in the separation of charged molecules.